中文摘要：

以龙眼品种红核子LC2胚性细胞系为材料，进行体细胞胚胎（简称体胚）发生及其同步化调控，获取体胚发生过程中各个主要发育阶段的培养物（胚性愈伤组织、球形胚、子叶形胚、早期成熟胚、中期成熟胚、晚期成熟胚），采用双向电泳对体胚发生过程中特异表达蛋白进行分析.结果表明：（1）龙眼胚性愈伤组织中，pI为4-5的酸性蛋白表达种类最多，在体胚发育的过程中逐渐消失，相反，pI为5-6的微酸性蛋白的表达逐渐增多；（2）体胚发育后期，尤其是成熟胚晚期，分子质量大的蛋白大量减少，分子质量较小的蛋白增多并逐渐积累；（3）虽然在各个发育阶段都会出现一些新的特异蛋白，但是没有改变蛋白质种类逐渐减少的趋势；（4）发现了一些有差异的特异蛋白点，尤其是成熟胚后期，有一系列大分子质量的蛋白消失，新出现了一部分分子质量较小的蛋白，其中几种表达量很大，可能与体胚发育有密切关系.

英文摘要：

In this experiment, the LC2 embryogenic cell line of the cultivar Honghezi of longan （ Dimocarpus longan Lout. ） was used as materials for obtaining the synchronized embryogenic cultures of somatic embryogenesis at different developmental stages （the embryogenic callus, globular embryo, cotyledonary embryo, early-stage mature embryo, mid-stage mature embryo and latestage mature embryo） for further 2-DE analysis of the proteins expressed specifically during somatic embryogenesis in longan. The 2-DE analysis showed that ： （ 1 ） the embryogenic callus of longan expressed the most varieties of acidic proteins （ the values of pI were between 4 and 5 ）, which eliminated gradually in the process of development of somatic embryos, on the opposite, slightly acidic proteins （ the values of pI were between 5 and 6） increased gradually; （2） during the late developmental stages, the high-molecular-weight （HMW） proteins eliminated gradually, and the low-molecular-weight （LMW） proteins simultaneously appeared and accumulated gradually; （3） although there were specific spots at each stage, the general diversificational trend was obviously decreased gradually; （4） there were some differential spots standing for specific proteins, especially at the late-stage somatic embryogenesis, a series of HMW proteins disappeared, and a series of LMW proteins appeared, which included some highly expressed proteins that were probably closely associated with somatic embryogenesis.