利用BIOLOG方法研究酸雨胁迫下接种外生菌根真菌对马尾松幼苗土壤微生物的缓解作用。采用盆栽方法,设置3种不同pH[5.6(CK),4.5,3.5]的模拟酸雨强度,分别设置彩色豆马勃接种和不接种的处理。结果表明:1)强酸胁迫可降低土壤微生物的活性、丰富度和多样性,降低土壤微生物对碳源的利用;2)弱酸的对照处理(pH5.6)下接种外生菌根真菌后可中和弱酸降水对土壤微生物的影响;3)强酸处理下接种外生菌根真菌能提高土壤微生物活性,提高丰富度和多样性,提高微生物对碳源的利用,且改变碳源利用结构。
This study aims to explore the effects of ectomycorrhizal colonization on soil microbial community and function of Pinus massoniana seedlings grown in acidified soils by using sole-carbon-source-utilization (BIOLOG). we set three levels of acid treatments, pH3.5, pH4.5 and pH5.6 (control), and applied two ectomyeorrhizal treatments (inoculated Pisolithus tinctorius, non-inoculated) in a pot experiments. Masson pine seedlings were inoculated with P, tinctorius (ECM) for 210 days in acidified forest soil originating from Chongqing, Southwest China. The results showed that: 1) Compared with the control treatment (pH 5.6) , high acid stress reduced the average vitality, soil microbial richness and diversity indices; 2) With the control treatment, ectomyeorrhizal fungi counteracted the effects of mild acid on soil microbial community; 3) With high acid stress treatments, ectomyeorrhizal fungi increased soil microbial activity, richness and diversity, and changed the utilization pattern of carbon source.