中文摘要：

目的：本研究在二氧化钛纳米管层表面固定了KRSR序列短链多肽，对此材料的粘附和诱导成骨的能力进行了研究。方法：于二氧化钛纳米管层上共价连接固定多肽后，对材料进行表征，并在试件上接种大鼠骨髓间充质干细胞，使用各种技术对细胞的粘附和分化进行评价。结果：固定KRSR的二氧化钛纳米管层对细胞的粘附率无明显影响，但铺展、骨向分化程度均优于对照组。结论：固定KRSR的二氧化钛纳米管层能够较好地诱导细胞骨向分化，有一定的临床应用价值。

英文摘要：

Objective： To functionalize TiO2 nanotube layers with KRSR and to examine the responses of rat mes- enchymal stem cells to this new material in vitro. Methods： SEM and AFM were used to characterize the materials. Tests were taken to characterize cells adhesion and osteogenic differentiation. Results： Immobilized KRSR increased mesenchymal stem ceils spreading on TiO2 nanotube layers. Moreover, osteogenic differentiation increased on the KRSR-modified TiO2 nanotube layers. Conclusion： KRSR--modified TiO2 nanotube layers have satisfactory bio- logical properties and should be further studied for improved orthopedic applications.