中文摘要：

目的：构建一种特殊的荧光素报告载体检测DNA甲基化对FCERIG基因启动子调控序列的调控活性。方法：用补丁甲基化技术构建特殊的含完全甲基化和模拟甲基化的FCERlG启动子调控序列的PGL-3荧光素报告载体；通过比较完全甲基化与模拟甲基化荧光素报告载体活性来检测DNA甲基化对FCERG基因启动子调控序列的调控活性。结果：成功构建特殊的完全甲基化与模拟甲基化FCERIG启动子调控序列荧光素报告载体；并检测出完全甲基化与模拟甲基化荧光素报告载体活性比为（0.36±0.07）：1（P〈0.001）。结论：FCERIG启动子调控序列是甲基化敏感位点，FCERIG启动子受DNA甲基化调控．

英文摘要：

Objective： To construct a special luciferase reporter to detect DNA methylation regulatory activity in FCER1 G gene promoter regulatory element. Methods： We constructed special full and mock methylated FCER1G gene promoter regulatory luciferase reporters by patch-methylation, and detected DNA methylation regulatory activity by comparing the luciferase activity of full-methylated luciferase reporters with mock-methylated reporters. Results： We successfully constructed the full and mock methylated FCERIG gene promoter regulatory luciferase reporters. The ratio of luciferase activity between the full methylated and themock methylated was （0.36±0.07）： 1 （P〈0.001）. Conclusion： FCER1G promoter activity is methylation-sensitive and is regulated by DNA methylation.