中文摘要：

目的：通过体外对大鼠跟腱成纤维细胞过度拉伸来模拟体育训练跟腱损伤后重塑的效应分子MMP-2和TIMP-1,2的响应和相关信号通路。方法：原代分离大鼠跟腱成纤维细胞,扩增后采用等双轴细胞力学拉伸装置对细胞进行：1）正常对照组;2）14%力学拉伸组;3）力学拉伸组并加入NF-κB信号通路抑制剂。拉伸后提取细胞上清液进行明胶酶谱法和Western Blotting检测MMP-2以及NF-κB磷酸化蛋白;并提取细胞总RNA,反转录后进行半定量PCR对TIMP-1,2进行检测。,然后对MMP-2,TIMP-1,2等进行基因水平和蛋白水平的检测。结果：1）力学损伤可使跟腱成纤维细胞MMP-2表达明显上升（P〈0.05）;2）力学损伤对跟腱成纤维细胞中TIMP-1,2的表达无显著性影响;3）力学损伤可引起NF-κB磷酸化蛋白表达明显上升（p〈0.05）;4）NF-κB抑制剂可明显抑制力学损伤引起的MMP-2过量表达（p〈0.05）。结论：体育运动引起的过度拉伸损伤可通过NF-κB上调MMP-2的表达和活性,提示过度拉伸引起的运动损伤可能与跟腱损伤和病变通过MMP直接相关。

英文摘要：

Aim： To determine the effect of in vitro injury on the expression of MMP-2, TIMP-1, and 2 as well as the underlying signal pathway in tendon fibreblasts. Methods： Tendon fibroblasts are achieved from primary cell culture from rat tendon tissues, after expansion we employ the equi-biaxial stretch chamber to load the cells with 1） control （0%）; 2） 14% injurious stretch; 3）14% injurious stretch with NF-κB inhibitors. After stretch, the conditioned mediums are extracted to analyze MMP-2 with zymography, NF-κB with western blot and TIMP-1, 2 with RT-PCR. Results： 1 ） mechanical injury will elevate the expression of MMP-2 in tendon fibroblasts （p 〈 0.05） ; 2） mechanical stretch can induce the expression of NF-κB （p〈 0.05）; 3） the inhibitor of NF-κB can significantly block the injury induced MMP-2 activities. Conclusion： mechanical injury can induce the expression of MMP-2 via NF-κB, which implies that the mechanical injury can directly induced the tendon injury and pathogenesis through MMPs.