中文摘要：

目的：从山莨菪根部的总生物碱提取物中分离乙酰胆碱酯酶抑制剂。方法：山莨菪根经95％乙醇提取、4mol／LHCl和4mol／LNaOH酸碱处理，氯仿萃取，得到总生物碱。采用薄层色谱生物自显影法跟踪分离目标组分，再用改良的Ellman法进一步测定抑制率。利用多种柱色谱分离得到3个化合物（1-3），通过^1 H-NMR、^13 C-NMR、ESI-MS波谱方法并与文献比对鉴定化合物的结构。结果：从山莨菪根部的总生物碱提取物中分离得到3个已知化合物，它们分别被鉴定为东莨菪碱，樟柳碱，阿托品，其乙酰胆碱酯酶抑制率分别为45．28％，38．08％和25．10％。结论：莨菪碱衍生物具有较弱的乙酰胆碱酯酶抑制活性。

英文摘要：

Objective ： To isolate acetylcholinesterase inhibitor from total alkaloidal extracted of the roots of Anisodus tan- guteus. Method ： The root of Anisodus tangutcus were extracted by 95 % ethanol and treated with 4 mol · L^-1HCl and 4 mol · L^-1 NaOH , and then extracted by chloroform to obtain total alkaloidal extracts. The bioassay -guided isolation of the AChE inhibitor was executed by TLC bioautographic method , and modified Ellman＇ s colorimetric method in 96 - well microplates. Three compounds （ 1 - 3 ） were obtainded by various column chromatographic methods and their struc- tures were elucidated based on1HNMR, 13 C - NMR , ESI - MS analysis and compared with literature reports. Result ： Three known compounds were isolated and identified as scopolamine, anisodine, atropine, respectively. Their inhibitory rates of 1 -3 against AchE were 45.28%, 38.08% and 25.10%, respectively. Conclusion： Derivatives of hyoseyamine have a weak inhibitory activity to AchE.