中文摘要：

目的探讨瞬时受体电位香草酸亚型1（TRPV1）通道对支气管哮喘大鼠气管平滑肌细胞增殖和炎症的影响。方法 2013年6月—2014年6月,选取5～6周龄清洁级健康雄性SD大鼠60只,采用随机数字表法分为：正常对照组、支气管哮喘组、支气管哮喘＋辣椒素（CAP）组、支气管哮喘＋辣椒卓平（CPZ）组,每组15只。支气管哮喘组、支气管哮喘＋CAP组、支气管哮喘＋CPZ组大鼠制作支气管哮喘模型,支气管哮喘＋CAP组大鼠给予含有0.01%CAP的饲料喂养,支气管哮喘＋CPZ组大鼠给予含有0.01%CPZ的饲料喂养。病理学检查显微镜下找到完整肺内支气管横断面,计算管壁面积（WA）/管腔内周长（Pi）、支气管平滑肌面积（S）/Pi、支气管平滑肌细胞核数（N）/Pi,采用实时荧光定量反转录聚合酶链式反应（RT-q PCR）和Western blotting法检测气管平滑肌组织TRPV1、增殖细胞核抗原（PCNA）mRNA及其蛋白表达水平,酶联免疫吸附试验（ELISA）法检测大鼠气管平滑肌组织白介素（IL）-4、IL-5、IL-13表达水平。结果支气管哮喘组和支气管哮喘＋CAP组大鼠气管WA/Pi、S/Pi、N/Pi高于对照组（P〈0.05）;支气管哮喘＋CAP组大鼠气管WA/Pi、S/Pi、N/Pi高于支气管哮喘组（P〈0.05）;支气管哮喘＋CPZ组大鼠气管WA/Pi、S/Pi、N/Pi低于支气管哮喘＋CAP组（P〈0.05）。支气管哮喘组和支气管哮喘＋CAP组大鼠气管平滑肌组织TRPV1、PCNA mRNA及其蛋白、IL-4、IL-5、IL-13表达水平高于对照组（P〈0.05）;支气管哮喘＋CAP组大鼠气管平滑肌组织TRPV1、PCNA mRNA及其蛋白、IL-4、IL-5、IL-13表达水平高于支气管哮喘组（P〈0.05）;支气管哮喘＋CPZ组大鼠气管平滑肌组织TRPV1、PCNA mRNA及其蛋白、IL-4、IL-5、IL-13表达水平低于支气管哮喘和支气管哮喘＋CAP组（P〈0.05）。结论 TRPV1、PCNA mRNA及其蛋白、IL-4、IL-5、IL-13在支气管哮喘大鼠气管平滑肌组织中高表

英文摘要：

Objective To observe how TRPV1 channel work on proliferation and inflammation in airway smooth muscle cells of asthmatic rat. Methods From June 2013 to June 2014,60 healthy clean male SD rats which were five to six weeks old were selected and randomly divided into control group,bronchial asthma group,asthma ＋ capsaicin（ CAP） group and asthma＋ capsazepine（ CPZ） group. Each group included 15 rats. Asthmatic models were established for bronchial asthma group,asthma＋ CAP group,asthma ＋ CPZ group. Asthma ＋ CPA group were fed with food which contains 0. 01% CAP,asthma ＋ CPZ group were fed with food which contains 0. 01% CPZ. Intact cross section of bronchus were observed under microscope,the pipe wall area（ WA） / lumen perimeter（ Pi）, ASM area（ S） / Pi, ASM nuclei number（ N） / Pi were caculated. TRPV1 and proliferating cell nuclear antigen（ PCNA） mRNA and protein expression levels in airway smooth muscle tissue were measured by real- time fluorescent quantitative reverse transcription polymerase chain reaction（ RT- q PCR） and Western blotting. Expression level of IL-4, IL-5 and IL-13 in airway smooth muscle tissue homogenate supernatant was measured by enzyme- linked immunosorbent adsorption test（ ELISA）. Results Compared with control group,rats in bronchial asthma group and asthma＋ CAP group had higher value in WA / Pi,S / Pi,N / Pi（ P〈0. 05）. Compared with bronchial asthma group,rats in asthma＋ CAP group had higher value in WA / Pi,S / Pi,N / Pi（ P〈0. 05）. Compared with asthma ＋ CAP group,rats in asthma ＋ CPZ group had lower value in WA / Pi,S / Pi,N / Pi（ P〈0. 05）. Compared with control group,bronchial asthma group and asthma＋ CAP group had higher expression level of TRPV1,PCNA mRNA and protein,IL-4,IL-5,IL-13 in airway smooth muscle tissue（ P〈0. 05）. Compared with bronchial asthma group,asthma ＋ CAP group had higher expression level of TRPV1,PCNA mRNA and protein,IL-4,IL-5,IL-13 in airway smooth muscle tissue（ P〈0. 05）. Compar