了解不同细胞或同类细胞在不同发育阶段,不同生理状态下的基因表达状况,可以为研究生命活动过程提供重要的信息。表达基因分析方法不断完善,包括抑制消减杂交技术,基因表达序列分析,基因表达谱芯片,电子克隆等。作者主要介绍了这些技术的原理、方法、特点及其应用情况。
Identifying changes in gene expression is of prime interest in molecular biology. A variety of methods have been developed for analyzing differentially expressed genes. There are several methods widely applied, including suppression subtractive hybridization (SSH), serial analysis of gene expression (SAGE), cDNA microarrays, silicon cloning and et al. This manuscript describe the principle, characteristic and application of these methods.