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中文摘要：
目的采用RP—HPLC法测定打箭菊中木犀草素的含量。方法色谱柱为Kromasil RP—C18（250mm×4．6mm，5μm）；流动相为甲醇-0．2％磷酸（60：40）；流速1．0ml·min^-1检测波长350nm；理论塔板数按木犀草素计应不低于1×10^4结果线性范围为0．106—1．060mg；线性回归方程为：Y=2．31×10^3X-10．82（r=0．9995）；平均回收率为96．8％，RSD：2．0％。结论所建方法操作简便、稳定、专属、重复性好，可用于藏药打箭菊的质量控制。
英文摘要：
OBJECTIVE To establish an RP - HPLC method for determination of luteolin in Flos Pyrethri tatsienensis. METHODS A Kromasil RP - C18（250 mm ×4.6 mm,5 μm） column was used. A mixture of methonal - 0.2% （v/v） phosphoric acid solution was used as mobile phase at the flow rate of 1.0 ml· min ^- 1 and the detective wavelength was 350 nm. RESULTS The peak areas and concentrations of luteolin showedagood correlation in the range of 0. 106 - 1. 060 mg with a correlation coefficient of 0. 9995. The linear equation was Y =2.31 ×10^3X - 10.82 and the average recovery was 96.8%. CONCLUSION The method has been proven to be simple, stable and repeatable, and can be applied for quality control of the Tibetan Medicine Flos Pyrethri Tatsienensis.
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