中文摘要：

骨组织不同细胞相互交织构成了复杂的网络结构，通过旁分泌和间隙连接途径相互传递信号，调节了个体发育的骨重塑和成体的骨重建过程。细胞共培养技术是研究细胞间相互作用的重要手段，目前主要有接触式和非接触式两种。就骨组织而言，由于骨细胞（osteocyte）在力学感知及调节骨重塑／重建平衡中的中心枢纽作用，建立适宜于骨细胞与骨组织其他细胞间的共培养技术，对于骨组织基础生物学的研究具有重要意义。该文就当前应用于骨组织生物学中的细胞共培养技术进行综述。

英文摘要：

Different bone cells constitutes a complex network structure, which transmit signals each other via paracrine and gap junction channels and adjust bone modeling and remodeling. Cells co-culture technique is the key method to investigate cell/cell interactions in vitro. Currently, this technique has two types： the contact coculture and non-contact co-culture. For the bone tissue, since the importance of osteocyte in mechanical sensing and in regulation of bone modeling and remodeling, it is important for the research of bone biology to establish the suitable co-culture technique between osteocyte and other bone cells. This article is aimed at reviewing the development of cells co-culture technique in the bone tissue biology.