中文摘要：

根据NCBI上已经报道的hog1序列，利用简并引物在线设计工具CODEHOP设计出两对简并引物，通过巢式PCR扩增，得到一段大小为635 bp的基因片段，将其克隆到T载体上并测序，将测得的序列在NCBI的Blast搜索发现，其与已报道的其他一些物种的hog1基因有同源性.用CODEHOP程序化设计简并引物可信性强，阳性率高.该基因的成功克隆为研究杜氏盐藻的HOG信号途径奠定了基础.

英文摘要：

According to the published sequence of hog1 from NCBI database, we designed the degenerate primers of hog1 using CODEHOP software. Using these degenerate primers, a partial hog1 cDNA of 635bp was amplified from Dunalilla salina and cloned into T vector. Sequence analysis showed that the cloned hog1 shares high sequence identity to those of hog1 from other species. The results indicated that the degenerate primers designed by the CODEHOP could be used to amplify specific gene fragment. Cloning of hog1 gene fragment would be helpful to further characterize the HOG signaling pathway of Dunalilla salina.