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Over-expression of Testis-specific Expressed Gene 1 Attenuates the Proliferation and Induces Apoptosis of GC-1spg Cells

ISSN号：1001-9030
期刊名称：《中华实验外科杂志》
时间：0
分类：Q253[生物学—细胞生物学] S852.33[农业科学—基础兽医学;农业科学—兽医学;农业科学—畜牧兽医]
作者机构：[1]First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China, [2]Department of Surgery, [3]Human Disease Related Gene Research Group, [4]Department of Pathology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
相关基金：This project was supported by grants from the National Natural Science Foundation of China （Nos. 30200284, 30600278, 30772359, 81100464, and 81200883）, Program for New Cen- tury Excellent Talents in University of China （No. NCET-06-0641）, Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry of China （No.1 2008-889）, the Youth Foundation of The First Affiliated Hospital of Zhengzhou University for Doctor of Medicine, and General Financial Grant from the China Postdoctoral Science Foundation of China （No. 2012M521410）.

作者：顾朝辉[1,2,3], 田凤艳[1], 普嘉瑞[2,3], 郑丽端[4], 梅红[2,3], 曾甫清[2], 杨锦建[1], 阚全程[1], 童强松[2,3,4]

关键词：细胞增殖, 诱导凋亡, 过表达, 特异表达基因, 睾丸, 特异性表达基因, 胱天蛋白酶, 真核表达载体, testis-specific expressed gene 1, apoptosis, gene expression

中文摘要：

The effects of over-expression of testis-specific expressed gene 1(TSEG-1) on the viability and apoptosis of cultured spermatogonial GC-1spg cells were investigated, and the immortal spermatogonial cell line GC-1spg(CRL-2053?) was obtained as the cell model in order to explore the function of TSEG-1. We transfected the eukaryotic vector of TSEG-1, named as pEGFP-TSEG-1 into cultured spermatogonial GC-1spg cells. Over-expression of TSEG-1 inhibited the proliferation of GC-1spg cells, and arrested cell cycle slightly at G0/G1 phase. Transfection of TSEG-1 attenuated the transcript levels of Ki-67, PCNA and cyclin D1. In addition, over-expression of TSEG-1 induced early and late apoptosis, and reduced the mitochondrial membrane potential of GC-1spg cells. Moreover, transfection of TSEG-1 significantly enhanced the ratio of Bax/Bcl-2 and transcript levels of caspase 9, and decreased the expression of Fas and caspase 8 in GC-1spg cells. These results indicated over-expression of TSEG-1 suppresses the proliferation and induces the apoptosis of GC-1spg cells, which establishes a basis for further study on the function of TSEG-1.

英文摘要：

The effects of over-expression of testis-specific expressed gene 1（TSEG-1） on the viability and apoptosis of cultured spermatogonial GC-1spg cells were investigated, and the immortal spermatogonial cell line GC-1spg（CRL-2053？） was obtained as the cell model in order to explore the function of TSEG-1. We transfected the eukaryotic vector of TSEG-1, named as pEGFP-TSEG-1 into cultured spermatogonial GC-1spg cells. Over-expression of TSEG-1 inhibited the proliferation of GC-1spg cells, and arrested cell cycle slightly at G0/G1 phase. Transfection of TSEG-1 attenuated the transcript levels of Ki-67, PCNA and cyclin D1. In addition, over-expression of TSEG-1 induced early and late apoptosis, and reduced the mitochondrial membrane potential of GC-1spg cells. Moreover, transfection of TSEG-1 significantly enhanced the ratio of Bax/Bcl-2 and transcript levels of caspase 9, and decreased the expression of Fas and caspase 8 in GC-1spg cells. These results indicated over-expression of TSEG-1 suppresses the proliferation and induces the apoptosis of GC-1spg cells, which establishes a basis for further study on the function of TSEG-1.

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