中文摘要：

第二代测序技术发展到一定阶段以后其缺陷逐渐显现，而第三代测序技术的出现在一定程度上弥补了第二代测序技术在应用方面的缺点。就目前正在发展的5种第三代高通量测序技术的原理进行了阐述，并比较了这几种测序技术的优缺点，最后对三代测序技术在基因组测序，甲基化研究，RNA测序以及医学方面的应用作了简单介绍。

英文摘要：

With the development and application of next generation sequencing technology （NGS）, some disadvantages are emerging. The third generation single molecular sequencing technology, to a certain extent, may offset these disadvantages of the next generation sequencing in application. This study elaborated the 4 sequencing principles of the third generation single molecular sequencing technologies, compared the advantages and disadvantages with these sequencing technologies, and presented their application in genome sequencing, methylation research, RNA sequencing and medical research.