以辐射诱变的线艺兰无菌丛芽为试材,设置不同BA、NAA浓度,研究了丛芽继代增殖、无根苗生根培养的快繁技术。结果表明:1/2MS+BA 2.0mg/L+NAA 0.5mg/L+香蕉泥80g/L对丛芽的增殖效果最好,增殖率为310%,1/2MS+NAA 1.0mg/L+0.1%活性碳诱导生根效果最好,生根率达到100%,且植株长势健壮,线艺艺性能够稳定遗传。
Taking Cymbidium with verge line pattern leaves induced by irradiation as test material,sterile buds were used as explants in tissue culture to study the influence of BA and NAA in different concentration,mashed bananas and activated carbon on their regeneration under 1/2MS basic medium.The results showed that 1/2MS+BA 2.0mg/L+NAA 0.5mg/L+mashed bananas 80g/L could make multiplication rate to be 310%.1/2MS+NAA 1.0mg/L+0.1%active carbon could make rooting rate to be 100%and plant grown healthily,while the verge line pattern leaves could be stable genetic.