欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
通过酸酐同系物标记蛋白质的N-末端，经过凝胶电泳分离，使用基质辅助激光解析电离飞行时间质谱仪进行鉴定，建立了一种基于化学标记和质谱技术的、用于蛋白质的，N-末端鉴定和相对定量新方法。标记后的蛋白质的Ⅳ端肽以相差14 Da的成对峰形式在质谱中出现，而非N-末端肽则是以单峰呈现，从而实现了末端肽的特异性识别；通过端肽的信号幔度对3种标记蛋白质的不同比例的混合溶液实现了相对定量分析。
英文摘要：
A new method was developed for quantifying proteins and identifying their N-termini using homolog labeling coupled with gel-based separation and MALDI-TOF mass spectrometry. Peak pairs with a mass difference of 14 Da were identified as N-terminal peptides while the internal peptides were single peaks and the ratios of their peak intensity were calculated for relative quantification of proteins. The results obtained from 3-protein mixtures demonstrate that this homolog labeling method enables the identification of N-terminal fragment ions from other fragments and quantitation of proteins simultaneously.
同期刊论文项目
同项目期刊论文
A method for rapidly confirming protein N-terminal sequences by matrix-assisted laser desorption/ io
Systematic characterization of the covalent interactions between epigallocatechin gallate and peptid
The miniaturization of bioanalytical assays and sample pretreatments by exploiting meso-fluidic lab-
Selective isolation of acidic proteins with a thin layer of multi-walled carbon nanotubes functional
One system with two fluorescence resonance energy transfer (FRET) assembles among quantum dots, gold
Fiber-optic fluorescence sensor for dissolved oxygen detection based on fluorinated xerogel immobili
Improved selection of LMW over HMW proteins from human plasma by mesoporous silica particles with ex
Isolation of hemoglobin from human blood by solid phase extraction with lanthanum(III) modified zeol
A pH-responsive poly(N-isopropylacrylamide -co-acrylic acid) hydrogel for the selective isolation of
Poly(phthalazine ether sulfone ketone) as novel stationary phase for stir bar sorptive extraction of
Biological cell-sorption for separation/preconcentration of ultra-trace cadmium in a sequential inje
New developments in flow injection/ sequential injection on-line separation and preconcentration cou
Selective extraction of low molecular weight proteins by mesoporous silica particles with modified i
Functionalization of multiwalled carbon nanotubes with hyperbranched polyethyleneimine for highly se
A highly fluorescent hydrophilic ionic liquid as potential probe for the sensing of biomacromolecule
A new poly (phthalazine ether sulfone ketone)-coated fiber for solid-phase microextraction to determ
Surface-assembly of graphene oxide nanosheets on SiO2 particles for the selective isolation of hemog
Combination of Improved 18O Incorporation and Multiple Reaction Monitoring: A Universal Strategy for
Coating a thin layer of NaBH4 solution for mercury vapor generation coupled to atomic fluorescence s
Direct and indirect immunolabelling of HeLa cells with quantum dots Direct and indirect immunolabell
Efficient discovery of abundant post-translational modifications and spectral pairs using peptide ma
Molecular bases of thioredoxin and thioredoxin reductase-mediated prooxidant actions of (-)-epigallo
N-glycosylation proteome of endoplasmic reticulum in mouse liver by ConA affinity chromatography cou
A simple and efficient frit preparation method for one-end tapered-fused silica-packed capillary col
Efficient Tryptic Proteolysis Accelerated by Laser Radiation for Peptide Mapping in Proteome Analysi
Facile Synthesis of Mercaptophenylboronic Acid-Functionalized Core-Shell Structure Fe3O4@C@Au Magnet
Novel Proteomic Strategy Reveal Combined Antitrypsin and Cathepsin D as Biomarkers for Colorectal Ca
Concanavalin A-immobilized magnetic nanoparticles for selective enrichment of glycoproteins and appl
SnO2@Poly(HEMA-co-St-co-VPBA) Core-shell Nanoparticles Designed for Selectively Enriching Glycopepti
N-terminal Cyclization of Peptides in Large-scale Protein Identification Based on Biological Mass Sp
Relationship between Sample Loading Amount and Peptide Identification and Its Effects on Quantitativ
Application of open tubular capillary columns coated with zirconium phosphonate for enrichment of ph
Nanozeolite-driven approach for enrichment of secretory proteins in human hepatocellular carcinoma c
Iterative Non-m/z-sharing Rule for Confident and Sensitive Protein Identification of Non-shotgun Pro
A Rapid Isolation and Identification Method for Blocked N-Terminal Peptides by Isothiocyanate-couple
Systematic characterization of the covalent interactions between (-)-epigallocatechin gallate and pe
Fragmentation and Site-Specific Quantification of Core Fucosylated Glycoprotein by Multiple Reaction
Preparation of polypyrrole- coated magnetic particles for micro solid-phase extraction of phthalates
Hydrazide-functionalized magnetic microspheres for the selective enrichment of digested tryptophan-c
Characterization of glycoprotein digests with hydrophilic interaction chromatography and mass spectr
期刊信息
位置：