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鸡胚原始生殖细胞(PGCs)玻璃化冷冻的研究
  • ISSN号:0366-6964
  • 期刊名称:《畜牧兽医学报》
  • 时间:0
  • 分类:S831.3[农业科学—畜牧学;农业科学—畜牧兽医]
  • 作者机构:[1]扬州大学动物科学与技术学院,扬州225009, [2]中国农业科学院家禽研究所,扬州225003
  • 相关基金:国家自然科学基金资助(30170678)
中文摘要:

对发育至第19期和第28期鸡胚性腺原始生殖细胞(Primordial germ cells,PGCs),用6种玻璃化冷冻液1:10%DMS0+10%EG+10%PVP,Ⅱ:20%EG+10%PVP,Ⅲ:20%DMSO+10%PVP,Ⅳ:10%DMSO+10%EG+0.5mol/L Surcose,Ⅴ:20%EG+0.5mol/LSurcose,Ⅵ:20%DMSO+0.5mol/L Surcose进行冷冻保存。结果:第19期鸡胚PGCs玻璃化冷冻复苏后存活率,在冷冻液Ⅱ与Ⅳ之间差异不显著(P〉0.05),Ⅴ与Ⅵ之间差异显著(P〈0.05),其余各冷冻液之间差异均极显著(P〈0.01)。第28期鸡胚PGCs玻璃化冷冻复苏后存活率,在冷冻液Ⅳ与Ⅴ之间差异显著(P〈0.05),Ⅲ与Ⅳ、Ⅴ之间差异不显著(P〉0.05),Ⅱ与Ⅲ、Ⅳ之间差异不显著(P〉0.05),其余各玻璃化冷冻液之间差异均极显著(P〈0.01)。复苏后接种培养传至第2代的鸡胚PGCs细胞,PAS染色、AKP染色呈阳性并保持完整的二倍体核型。

英文摘要:

Six kinds of vitrification germ cells (PGCs) from gonads at freezing medias were used to cryopreserve isolated primordial stage 19 and stage 28 by ficoll density-gradient centrifugation. The six kinds of vitrification freezing medias were following: Ⅰ : 10%DMSO+10%EG+10% PVP, Ⅱ : 20% EG + 10% PVP, Ⅲ : 20% DMSO + 10% PVP, Ⅳ: 10% DMSO + 10% EG + 0. 5 mol/L surcose, Ⅴ :20%EG+0.5mol/L surcose, Ⅵ :20%DMSO+0.5 mol/L surcose. The viabilities of PGCs were performed after thawing by way of staining with trypan blue. The results showed: For the viability of the frozen-thawed PGCs at stage 19, it showed significant difference (P〈0.05)between the viability under freezing media Ⅵ and the viability under freezing media Ⅴ , and showed insignificant difference (P〉0.05)between the viability under freezing media Ⅱ and the viability under freezing media Ⅳ. But it showed very significant difference(P〈0.01) among the viabilities under the other freezing medias. For the viability of the frozen-thawed PGCs at stage 28, it showed significant difference(P(0.05)between the viability under freezing media Ⅳ and the viability under freezing media Ⅴ , and showed insignificant difference (P〉0. 05) among the viability under freezing media Ⅲ, Ⅳand Ⅴ , and so were the viabilities under freezing media Ⅱ , Ⅲ and Ⅳ (P〈0.05). But it showed very significant difference(P〈0.01)among the viabilities under the other freezing medias. The second passage PGCs cultured after thawing were positive to PAS staining and AKP staining and still kept intact karyotypes.

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期刊信息
  • 《畜牧兽医学报》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学技术协会
  • 主办单位:中国畜牧兽医学会
  • 主编:文杰
  • 地址:北京海淀区圆明园西路2号中国农科院畜牧所
  • 邮编:100193
  • 邮箱:xmsyxb@263.net
  • 电话:010-62815987 62816996
  • 国际标准刊号:ISSN:0366-6964
  • 国内统一刊号:ISSN:11-1985/S
  • 邮发代号:82-453
  • 获奖情况:
  • 1992年北京优秀期刊奖,1998年、2000年在全国畜牧兽医优秀期刊评比中获一等奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,美国剑桥科学文摘,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),瑞典开放获取期刊指南,中国北大核心期刊(2000版)
  • 被引量:21857