中文摘要：

为了解盐生杜氏藻（Dunaliella salina）烯醇酶（Enolase）在渗透耐受中的具体功能,利用基因组步行方法和巢式PCR,从D.salina中克隆了烯醇酶基因DsENO 5’上游约2 000 bp的调控序列,并对其进行序列分析.分析表明,它包含多个与转录调控有关的保守序列（如CAAT-box,TATA-box）,富含光﹑干旱及其它胁迫应答元件.利用实时荧光定量PCR的方法,研究了高渗﹑高温以及低温外界胁迫条件下DsENO的转录情况,发现其受高渗强烈抑制,高温显著诱导而低温微弱诱导.

英文摘要：

With the genome walking method and nested PCR,enolase gene DsENO 5＇upstream regulatory sequence（about 2 000 bp） was cloned from Dunaliella salina,and its sequence analysis was made to investigate the specifi c function of D.salina during osmotic stress tolerance.The result shows that it includes several conserved sequences related to transcriptional regulation,such as CAAT-box,TATA-box,and it is rich in light,drought and other stress responsive elements.With the real-time fluorescence quantitative PCR method,the DsENO transcription under hyperosmotic,high-temperature and low-temperature stress were studied to fi nd that it was strongly inhibited by hyperosmotic stress and significantly induced by high-temperature,but slightly induced by low-temperature.