中文摘要：

应用RT-PCR方法从鸭源Ⅰ型副黏病毒DP1/02株中扩增F基因并克隆入pMD18-T载体,经序列测定、分析,DP1/02株F基因与鸡新城疫病毒（NDV）国家标准强毒F48E9株的核苷酸、氨基酸序列同源性均达到99%。随后将F基因克隆入真核表达载体pCI-neo,构建真核表达质粒pCI-F,将阳性质粒体外转染Vero细胞,通过间接免疫荧光对真核质粒的表达进行鉴定,利用pCI-F质粒进行动物试验。结果表明构建的真核表达质粒pCI-F能够在Vero细胞中表达,雏鸭免疫14d后在体内可检测到特异性抗体,二次免疫雏鸭后,对NDV强毒的攻毒保护率为73%。本试验为利用F基因构建的核酸疫苗提供了重要的技术支持。

英文摘要：

Fusion glycoprotein（F） gene of paramyxovirus strain DP1/02 isolated from duck was amplified by reverse transcriptase polymerase chain reaction（RT-PCR） and cloned into pMD18-T vector.The sequence analyses of F gene indicated that the homology of nucleotide acid sequences and amino acid sequences between DP1/02 strain and F48E9 strain,standard virulent strain of Newcastle disease virus F48E9 was 99%.Then,F gene was cloned into the eukaryotic expression vector pCI-neo.The recombinant plasmid pCI-F which was transferred into Vero cells successfully expressing F protein was identified by indirect immunofluorescence.In animal experiment,the ducks were vaccined by plasmid pCI-F,the result showed that the plasmid pCI-F was effective,for the first immunization stimulating duck to produce specific antibody 14 days later.After the second immunization,the specific antibody could protect 73% of the ducks against NDV standard virulent infection.The results of animal experiment suggested that to constructed eukaryotic plasmid which expressed F gene is useful for the development of nucleate vaccine.