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中文摘要：
通过对太子参总皂苷提取溶剂、药材颗粒度、料液比、提取时间、显色系统5个单因素考察,初步建立供试液的制备方法及检测时的显色条件,并利用正交设计试验优化供试液的制备工艺.结果表明：太子参粉末（过药典7号筛）以乙醇为提取溶剂,料液比为0.1 g∶20 mL,超声提取120 min所得供试液,用5％香草醛-冰醋酸溶液和60％硫酸溶液于560 nm处进行显色测定时,总皂苷的含量测定结果最优.该测定方法经精密度、稳定性、重复性、加样回收率试验考察后RSD＜ 3％,稳定可靠,可用于太子参皂苷含量的测定.
英文摘要：
The mensuration condition （inspecting solvent extraction,solid-liquid ratio,partical size,extraction time and color conditions） of total saponins conten in Radix Pseudostellariae were preliminarily established by the single factor experiment.Then the method of preparing testing solution was optimized by the orthogonal test.The results showed that when the solid-liquid ratio of Radix Pseudostellariae powder and ethanol was 0.1 g∶ 20 mL,the time of ultrasonic extraction was 120 min,the color conditions were 5％ vanillinacetic acid and 60％ sulfuric acid,the result of determination was optimum.After the tests of precision,stability,repeatability and sample recovery rate,RSD ＜ 3 ％,the method of determination was stable and reliable,and can be used to detecte total sapoins in Radix Pseudostellariae.
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