中文摘要：

利用杆状病毒表达系统,将病毒源性趋化因子病毒类白细胞介素8(vIL-8)基因克隆入转移载体pFastBacI,然后转化到DH10Bac感受态细胞中与Bacmid杆状病毒穿梭载体进行转座重组,通过重组子转染Sf9细胞得到含vIL-8的重组病毒rBacvIL-8。间接免疫荧光试验和雏鸡T、B淋巴细胞生物趋化分析结果表明,vIL-8在昆虫细胞中得到了表达,重组vIL-8在一定浓度范围内,可使鸡T、B细胞发生趋化作用,最高趋化指数分别为3.31±0.50和4.02±0.48,且是典型剂量依赖的钟形趋化性反应曲线;进一步分析发现vIL-8对B细胞趋化活性较对T细胞强?P<0.05),提示vIL-8对靶细胞的趋化性与病毒感染过程相关,在早期溶B细胞感染中有重要作用。

英文摘要：

In this study,vIL-8 gene was cloned into pFastBacI to get the recombinant baculovirus shuttle vector(Bacmid-vIL-8)by transforming DH10Bac competent cells.Then Sf9 insect cells were transfected with the recombinant Bacmid-vIL-8 DNA.Finally the recombinant virus that contained vIL-8 gene was obtained.The recombinant products of vIL-8 in IFA were expressed in Sf9 cells infected with the recombinant virus.Our results showed that the recombinant vIL-8 was able to attract chicken T and B lymphocytes in chemotaxis...