中文摘要：

目的：从受有机磷农药污染的土壤中分离能降解DDVP的菌株,对其进行鉴定和降解特性研究。方法：采用DDVP为惟一碳源和能源的无机盐培养基,通过富集培养、平板划线分离得到一株优势菌,编号为DDW-1,采用形态学、生理生化和16S-rDNA序列分析对其进行鉴定,采用气相色谱测定菌株DDW-1对DDVP的降解能力,并进行底物广谱性测试和降解酶定位实验。结果：该菌株鉴定为甲基杆菌属（Methylobacterium sp.）。降解特性试验结果表明,其最佳生长条件为温度28℃,初始pH为7.0,在该条件下,500mg·L^-1DDVP经过DDW-1菌株代谢3d后,降解率达63.7%。结论：菌株DDW-1能降解DDVP,该菌株产胞内酶。

英文摘要：

Objective：A DDVP-degrading bacterium was isolated from the long-term organophosphorous pesticide-polluted soil. Then the bacterium was identified and its degrading capability was studied. Method： Utilizing DDVP as the sole carbon and energy source, a predominated bacterium,designated as strain DDW-1,was isolated by enrichment culture and streak plate method.The strain DDW-1 was identified based on 16S rDNA sequence analysis and its morphological and physiological characteristics and its DDVP degradation capability was measured by gas chromatography. Result： The strain DDW-1 was identified as Methylobacterium sp. The optimum conditions for the growth of the strain DDW-1 were initial pH 7.0 and 28℃. After 3 days, DDVP was degraded by 63.7%under the optimum condition. Conclusion： DDVP could be degraded by strain DDW-1 and some endoenzyme was produced during this process.