介绍一种制备微小铁葡聚糖颗粒以及利用微小铁葡聚糖颗粒分离纤毛虫接合对的方法,通过本方法可以获得接合率达95%以上,且发育基本同步的实验材料。
In this report, methods for preparing magnetic iron-dextran particles and separating highly synchronized (95%) ciliate conjugating pairs are introduced. When iron-dextran particles are added to the mixtures of complementary mating types of ciliates, single cells and loose conjugating pairs form food vacuoles containing the irondextran particles while absent from the tight mating pairs. By applying magnetic force, single cells and loose mating pairs can be removed from the tight conjugating pairs. ~is method has several advantages: simplicity, speediness, high quality, universality and practicality. No negative effects have been found when perform the experiments by this method.