中文摘要：

本实验旨在对水牛成纤维细胞生长因子10（FGF10）基因进行克隆、生物信息学分析，同时探讨其在不同组织中的表达模式。以水牛卵巢的总RNA为模板，通过RT—PCR方法克隆得到FGFIO基因CDS区全长642bp，编码213个氨基酸。多重序列分析显示，水牛阳门0基因核苷酸序列与黄牛、羊、猪、人、小鼠同源性分别达到了99％、99％、93％、91％和89％。系统进化树分析表明，水牛和黄牛亲缘性最近，并且在不同物种进化过程中具有高度保守性。QRT—PCR结果表明，FGF10在睾丸中的表达量最高，卵巢和脾脏次之，肝脏和心脏中最低。本研究成功克隆水牛阳FIO基因，并检测其在水牛不同组织中的差异表达，为研究FGFIO基因在水牛卵泡发育过程中的作用奠定基础。

英文摘要：

The aim of this study was to perform the cloning and bioinformatics analysis of buffalo FGFIO gene, and then to investigate its expression profile in different tissues. The buffalo FGFIO gene fragment including a 642 bp whole length CDS（coded 213 amino acids）was cloned by RT-PCR. The results of multiple sequence alignment showed the nucleotide sequence of buffalo FGFIO gene shared 99%, 99%, 93%, 91% and 89% homologous with Bos taurus, Ovis ariessis, Sus scrofa, Homo sapiens and Mus musculus, respectively. Phylogenetic tree analysis indicated that buffalo had the closest relationship with Bos Taurus in evolution, and the evolutionary process was highly conservative in different species. QRT-PCR results revealed that the expression levels of FGFIO genewere diverse from each other in buffalo tissues, with the most abundant expression in testis, followed by spleen and ovary, less observed in heart and liver. In a word, the successful cloning and analysis of the FGFIO gene provides the foundation for further exploring its role in the development of buffalo follicles.