中文摘要：

目的：研究脉冲电磁场（PEMFs）对大鼠成骨细胞的作用。方法：取新生SD大鼠头盖骨，通过骨组织块培养法分离、培养成骨细胞。取传代第3代细胞分为5组，1—70组、2—70组、3—70组、4—70组、对照组，其中前4组为作用组，接受频率为70Hz，峰值磁场分别为1、2、3、4mT的PEMFs作用，对照组不接受PEMFs作用。按照分组剂量进行离体PEMFs作用（0．5h/d，连续作用4d），作用结束后，对各组分别进行细胞碱性磷酸酶（ALP）活性，核结合因子α1（cbfα1）表达，体外成骨能力指标-骨结节形成能力检测。结果：经PEMFs作用后，与对照组相比，成骨细胞ALP活性降低，cbfα1表达增多，骨结节数量显著增多。结论：PEMFs作用成骨细胞可促进成骨细胞功能分化，增强成骨细胞的细胞外基质矿化。

英文摘要：

Objective： To study the effect of pulsed electromagnetic fields （PEMFs） on rat osteoblasts. Methods： Osteoblasts were separated from newly born SD rat calvaria and cultured in vitro. The third-generation cells were divided into five groups including 1-70 group, 2-70 group, 3-70 group, 4-70 group and control group. According to the given dose of each group, treated groups were exposed to PEMFs （1, 2, 3 and 4 roT）, and control group was given nothing. After the treatments （0.5 h/d for 4 d）, the alkaline phosphatase （ALP） level, core-binding factor a1 （cbfa1/RUNX2） staining and mineralized nodules staining were detected respectively in five groups. Results： After exposure to PEMFs, ALP activity was significantly reduced in osteoblasts than that of control group. The cbfa1-positive osteoblasts were increased. And the number of mineralized nodules was significantly increased. Conclusion： PEMFs promote osteoblast differentiation to extracellular matrix maturation. PEMFs are contributed to osteoblast extracellular matrix mineralization.