中文摘要：

目的建立C57BL／6j小鼠人神经母细胞瘤移植瘤模型。方法取对数生长期SK—N—SH细胞1×10^7个ml，分别接种3d、7d、3周、4周、5周、6周、7周C57BIM6j系小鼠及6周BALB／C系裸鼠，观察小鼠成瘤率、相对肿瘤体积（RTV）、相对肿瘤增长速率（K）、相对肿瘤倍增时间（Td）、宿主存活时间及移植瘤病理学情况。结果C57BL／6j各年龄段及BALB／C持续成瘤率分别为：100％、100％、90％、70％、60％、0％、0％、100％。3周龄C57BL／6i与BALB／C比较RTV、K、．rd及存活时间均差异无统计学意义（P〉0．05）。结论在C57BIM6j小鼠体内成功建立人类异种移植瘤模型，3周龄为最适宜接种时间点。

英文摘要：

Objective To investigate the feasibility of human neuroblastoma xenograft model in C57BL/6j mice. Methods Three-day, 7-day, 3-week, 4-week, 5-week, 6-week and 7-week age C57BL/6j and 6-week age BALB/C nude mice were subcutaneously inoculated with 1×10^7 cell/ml SK-N- SH cells in logarithmic phase, respectively. The tumor generation rate, relative tumor volume （RTV） , relative growth rate （K） , relative doubling time （Td）, survival time and pathological changes were tested. Results The continuing tumor generation rate of C57BL/6j mice and BALB/C nude mice was 100% （ 10/ 10） at the 3rd day, 100% （10//10） at the 7th day, 90% （9/10） at the 3rd week and 70% （7/10） at the 4th week, 60% （6/10） at the 5th week, 0% （0/10） at the 6th week, 0% （0/10） at the 7th week, and 100% （10/10） at the 6th week, respectively. There was no significant difference in RTV, K, Td, and survival time （P 〉 0. 05 ） between 3-week age C57BL/6j mice and BALB/C nude mice. Conclusion Human neuroblastoma xenograft models were successfully established the in C57BL/6j mice, and 3-week age was the most appropriate opportunity of vaccination.