中文摘要：

从所采集柴油污染土壤样品中富集、分离得到柴油降解优势菌株,命名为B-3和B-4。根据其生理生化性质及16S rDNA序列比对分析,确定2株菌分别属于Tetrathiobacter kashmirensis、假单胞菌属（Pseudomonassp.）。由于实验中,B-3的生长曲线较特殊,故以B-3和典型石油烃降解菌假单胞菌属B-4为研究对象,进行柴油降解实验。将其分别在浓度为3.78 g/L的柴油培养基中培养,菌株B-3和B-4的降解率依次为60.98%和37.18%,其形态学观察、生理生化实验及生长曲线的测定结果也表明,B-3的生物学性质也明显高于B-4,且是新确定的一个新属新种,并且在柴油降解方面的研究很少。本研究结果可为柴油污染环境的生物修复提供一定的理论依据。

英文摘要：

The biodegradation bacteria were enriched and isolated from the diesel contaminated soil.Two dominant strains were obtained and named as B-3,B-4.These strains were identified as Tetrathiobacter kashmirensis and Pseudomonas sp.through morphological observation and 16S rDNA sequence analysis,respectively.The degradation capabilities of strain B-3 and strain B-4 were studied in this paper.The strains B-3 and B-4 were taken in the culture medium,in which diesel concentration was 3.78 g/L.The degradation rate of them was 60.98% and 37.18%,respectively.And the results of morphological observation and growth curve measurement to strain B-3 showed that the degradation capability is obviously higher than B-4.To our knowledge,this is also the newly established species about biodegrading diesel contaminated soil using strain B-3.