中文摘要：

研究免疫共刺激分子CD40在结肠癌细胞株的表达及重组可溶性人CD40配体（rshCD40L）对结肠癌细胞株的体外抑制作用。采用流式细胞仪分别检测4株结肠癌细胞（HCT116、Caco-2、SW48和COLO-320）CD40分子的表达,同时利用流式细胞仪检测γ干扰素（IFN-γ）对结肠癌细胞株CD40表达的影响。MTT法检测rshCD40L对结肠癌细胞的抑制作用,TUNEL法检测rshCD40L对结肠细胞的促凋亡作用,同时检测联合rshCD40L和IFN-γ抗结肠癌作用。结果流式细胞仪检测有3株结肠癌细胞（HCT116、Caco-2和SW48）表达CD40分子,IFN-γ能增强CD40＋结肠癌细胞CD40分子的表达。rshCD40L能明显抑制CD40＋结肠癌细胞的增殖作用（抑制率分别为HCT116：33.5%±5.5%;Caco-2：21.5%±3.6%;SW48：30.1%±4.2%）,而对CD40-结肠癌细胞株（COLO-320）无明显抑制作用（P〈0.05）。rshCD40L能诱导CD40＋结肠癌细胞的凋亡作用（凋亡细胞百分比为HCT116：25.6%±4.52%;Caco-2：15.71%±2.27%;SW48：18.0%±3.7%）,而对CD40-结肠癌细胞株（COLO-320）无诱导凋亡作用（P〈0.05）。另外,IFN-γ能明显增强rshCD40L对结肠癌细胞的抑制增殖和促凋亡作用。重组人CD40配体与IFN-γ联合可显著诱导CD40阳性的结肠癌细胞凋亡,抑制其增殖,具有潜在的抗肿瘤作用。

英文摘要：

To investigate the expression of CD40 molecule on colon cancer cell lines and to evaluate the growth-inhibitory effect of recombinant soluble human CD40L （rshCD40L） on colon cell lines,the expression of CD40 on colon cell lines （HCT116,Caco-2,SW48,and COLO-320） and the changes induced by interferon-γ（IFN-γ） were examined by flow cytometry.The growth-inhibitory activity of rshCD40L on colon cancer cell lines was examined by MTT assay and the proportion of apoptotic tumor cells was examined with TUNEL assay. Also,the growth-inhibitory effect of the combined activity of rshCD40L and IFN-γ was examined.As demonstrated by flow cytometry,the CD40 molecules could be detected on 3 strains of colon cancer cell lines （HCT116,Caeo-2 and SW48） and the molecular expression of CD40 on these cell lines could be enhanced by the action of IFN-γ.However,the proliferative activities of these cell lines were markedly inhibited by rshCD40L molecule with the growth-inhibitory activities of 33.5±5.5% for HCT116;21.5±3.6% for Caco-2 and 30.1±4.2% for SW48 cell lines respectively. Nevertheless,no apoptosis-induced effect could be detected in another CD40＋colon cancer cell line COLO320 （P0.05）.In addition,IFN-γ could significantly enhance the activities of proliferation inhibition and induction of apoptosis. From these observations,it is evident that the combined activity of rshCD40L and IFN-γ inhibit proliferation and induce apoptosis of the CD40＋colon cancer cell line,and thus has a potential anti-tumor effect.