中文摘要：

鼻咽的癌(NPC ) 是在南部的中国和东南亚洲的一个通常发生的肿瘤。当前的学习集中了于为 NPC 房间线 HNE1 的外部、不可分的蛋白质开发一个广泛的分析方法。外部膜蛋白质被 biotinylated 丰富提取， 0.1 M Na ₂ 公司 ₃, 和 H ₂ O。不可分或全部的血浆膜部分用 30% Percoll 密度等级 centrifugation 被准备与或没有 0.1 M Na ₂ 公司 ₃ 处理并且由西方的污点分析评估了。蛋白质受到与双人脚踏车团 spectrometry，与双人脚踏车团 spectrometry 相结合的钠 dodecyl sulfate-polyacrylamide 胶化电气泳动，和猎枪分析相结合的二维的电气泳动。我们分别地从外部、不可分、全部的血浆膜部分识别了 371， 180，和 702 蛋白质。总共， 848 非冗余的蛋白质(534 个组) 被识别。有约束力、催化、结构的分子是主要的班。除了 NPC 房间的已知的房间表面标记，分析在癌症房间是特征的疾病小径揭示了涉及多重发信号房间的小径的 311 蛋白质和 25 蛋白质。由寻找，差别表示了蛋白质数据库(http://protchem.hunnu.edu.cn/depd/index.jsp ) ， 199 蛋白质被发现是在以前的癌症 proteome 研究表示的差别。671 蛋白质蛋白质相互作用网络被获得，包括在这个工作的 178 识别蛋白质。五蛋白质的血浆膜本地化被免疫学的技术证实，验证这 proteomic 策略。我们的学习能为理解 NPC 的分子的机制提供一些帮助。

英文摘要：

Nasopharyngeal carcinoma （NPC） is a commonly occurring tumor in southern China and Southeast Asia. The current study focused on developing an extensive analysis method for the peripheral and integral proteins of NPC cell line HNE1. The peripheral membrane proteins were extracted by biotinylated enrichment, 0.1 M Na2CO3, and H20. Integral or total plasma membrane fractions were prepared using 30% Percoll density grade centrifugation with or without 0.1 M Na2CO3 treatment and evaluated by Western blot analysis. The proteins were subjected to two-dimensional electrophoresis combined with tandem mass spectrometry, sodium dodecyl sulfate-polyacrylamide gel electrophoresis combined with tandem mass spectrometry, and shotgun analysis. We identified 371, 180, and 702 proteins from peripheral, integral, and total plasma membrane fractions, respectively. In all, 848 non-redundant proteins （534 groups） were identified. Binding, catalytic, and structural molecules were the major classes. In addition to the known cell surface markers of NPC cells, the analysis revealed 311 proteins involved in multiple cell-signaling pathways and 25 proteins in disease pathways that are characteristic of cancer cells. By searching the Differentially Expressed Protein Database（http：//protchem.hunnu.edu.cn/depd/index.jsp） ,199 proteins were found to be differentially expressed in previous cancer proteome research. A 671 protein-protein interaction network was obtained, including 178 identified proteins in this work. The plasma membrane localization of five proteins was confirmed by immunological techniques, validating this proteomic strategy. Our study could offer some help for understanding the molecular mechanism of NPC.