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中文摘要：
新城疫病毒Ⅰ系苗Mukteswar株为中等毒力,野外分离株JS/7/05/Ch的基因组核苷酸与其具有高于99%的相似性,但其毒力却明显强于前者。为了给基因Ⅲ型NDV的遗传进化机制研究提供基础,本研究成功构建了JS/7/05/Ch株的反向遗传平台。首先根据GenBank上公布的基因Ⅲ型新城疫病毒JS/7/05/Ch株基因组全序列设计并合成8对引物,RT-PCR扩增目的片段后,分别依次亚克隆至pCR2.1载体中构建含有JS/7/05/Ch全长基因组cDNA的克隆质粒pJS/7/05/Ch,然后再通过特异性酶切位点将JS/7/05株全长基因组cDNA转移到TVT7R（0.0）转录载体中,成功构建出含JS/7/05/Ch株基因组全长cDNA的转录质粒pTVT/JS705。然后将该质粒与3个辅助质粒pCI-NP、pCI-P和pCI-L共转染BSR-T7/5细胞,转染60h后将该细胞及其上清接种鸡胚;血凝（HA）试验和RT-PCR结果表明JS/7/05/Ch株新城疫病毒被成功拯救。
英文摘要：
The genome of JS/7/05/Ch isolate shared more than 99% nucleotide identity with that of Mukteswar strain.However,the pathogenicity of JS/7/05/Ch was much stronger than that of Mukteswar strain.In order to provide a good foundation for the further related research,we built the rescue system of JS/7/05/Ch in this study.Based on the genomic sequence of JS/7/05/Ch strain,eight pairs of primers were designed to amplify the genomic fragments by RT-PCR and cloned into pCR2.1vector,to construct the plasmid pJS/7/05/Ch which contained the full-length cDNA of NDV.Then the full-length cDNA was transferred to TVT7R（0.0）vector to construct full-length NDV infectious clone,pTVT/JS705,using specific enzymes.The infectious clone together with three helper plasmids（pCI-NP,pCI-P and pCI-L）were cotransfected into BSR-T7/ 5cell, and the transfection supernatant was inoculated into SPF embryonated eggs to rescue the virus.The results of HA and RT-PCR indicated that JS/7/05/Ch strain of NDV was successfully rescued.
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