中文摘要：

目的：观察奇正消痛贴膏（qizheng pain-relieving plaster,QZPRP）对慢性炎症痛过程中背根神经节（dorsal root ganglion,DRG）和脊髓背角免疫活动异常的影响。方法：成年SD大鼠足跖注射完全弗氏佐剂（complete freund＇s adjuvant,CFA）建立炎性痛模型。建模后3 d患侧足跖分别包扎QZPRP。用up-down方法和Plantar test分别检测机械痛敏和热痛敏。用ELISA,免疫荧光法和Western blot检测免疫活动。结果：足底使用QZPRP可明显抑制CFA引起的机械痛敏和热痛敏,并促进水肿的恢复,而单纯使用溶剂和药粉无效。QZPRP抑制CFA引起的DRG巨噬细胞浸润、脊髓背角小胶质细胞和星形胶质细胞的激活、DRG和脊髓背角TNF-α上调和SFKs磷酸化。注射CFA上调DRG和脊髓背角的IL-10,QZPRP使其进一步升高。结论：皮肤施加QZPRP可通过抑制痛觉通路的异常免疫活动发挥抗炎镇痛作用,其免疫调节的机制尚有待研究。

英文摘要：

Objective： To investigate the effect of qizheng pain-relieving plaster（QZPRP）on the immune abnormality in dorsal root ganglion（DRG） and spinal dorsal horn produced by chronic inflammatory pain. Methods： The inflammatory pain model was established by injection of complete freund＇ s adjuvant（CFA） into foot plantar of adult SD rats. QZPRP（vehicle and powder）, vehicle or powder was applied to the ipsilateral foot 3d after injection. Up-down method and Plantar test were used to measure the 50% paw withdrawal threshold（PWT） to mechanical stimuli and paw withdrawal latency（PWL） to thermal stimuli, respectively. ELISA, immunohistochemistry, and Western blot were used to assess the expression of tumor necrosis factor-alpha（TNF-α）, interleukin-10（IL-10） and macrophage infiltration and activation of glial cells. Results： The mechanical allodynia, thermal hyperalgesia and foot swelling induced by injection of CFA were significantly attenuated by QZPRP but not by vehicle or powder applied onto injected foot. Mechanistically, skin application of QZPRP inhibited the CFA-induced infiltration of macrophage in DRG and activation of microglia and astrocytes in spinal dorsal horn, and the upregulation of TNF-αand phosphorylation of Src-family kinases in DRG and spinal dorsal horn resulting from CFA injection. IL-10 was upregulated in DRG and in spinal dorsal horn by injection of CFA, QZPRP further increasedthe expression of the anti-inflammatory cytokine. Conclusion： QZPRP may attenuate the inflammatory pain by inhibition of neuroinflammation. Further studies are needed for understanding the mechanisms underlying the regulation of the immune activity in DRG and in spinal dorsal horn by skin application of QZPRP.