用氯化铵和曝气自来水设置0(对照)、40、80、120、160、200、240 mg/L共7个氨氮质量浓度梯度,进行96 h的氨氮对(12±1.6)g合浦绒螯蟹(Eriocheir hepuensis)存活和摄饵影响的急性毒性实验。结果显示:96 h40 mg/L氨氮质量浓度组蟹存活率(100%)与对照组(0 mg/L)差异不显著,但80 mg/L以上质量浓度组与对照组(0 mg/L)蟹存活率差异显著(P〈0.05)。24、36、48、60、72、84、96 h,氨氮对合浦绒螯蟹的LC50值分别为405.889、280.942、245.845、211.232、177.588、162.561、134.089 mg/L,安全质量浓度为13.409 mg/L。120mg/L组的蟹存活时间与对照组差异不显著(P〉0.05),但与160 mg/L组蟹的存活时间差异显著(P〈0.05)。对照组与40 mg/L以上质量浓度组相对摄饵量有显著差异(P〈0.05)。
A total of six ammonia-N treatments( 40,80,120,160,200,240 mg / l) and a control( no ammonia-N added) were set up with ammonium chloride and artificial seawater for the acute ammonia-N toxicity experiment on survival rate and food intake of Eriocheir hepuensis. The mean body mass of crab was about( 12 ± 1. 6) g,and the experiment was for 96 h. The results showed that Eriocheir hepuensis can survive( 100%) for 96 h in the 40 mg / L of ammonia nitrogen concentration and below,which was significant different from that of the 80 mg / L group and over,( P 〈0. 05). The LC50 of ammonia on the crab at 24,36,48,60,72 h,84 and 96 h were 405. 889,280. 942,245. 845,211. 232,177. 588,162. 561 and 134. 089 mg / L. And the safe concentrations of ammonia nitrogen was 13. 409 mg / L. Crab's surviving time of 120 mg / L group( or the below) was not significant control group different significant from( P 〈0. 05),but significant different( P 〈0. 05) from 160 mg / L group and over. The relative food intake of crab of control group and concentration over 40 mg / L groups showed significant differences( P 〈0. 05).