中文摘要：

[目的]发掘海涂互花米草耐盐、水稻高产种质资源,创新互花米草与水稻正反远缘杂交适用方法。[方法]正交：水稻♀温汤杀雄剪颖授粉法、热气杀雄吸花授粉法;反交：互花米草♀剪颖去雄授粉法、连续去雄授粉法、温汤杀雄连续去雄授粉法。[结果]水稻♀热气去雄吸花授粉法比温汤杀雄剪颖授粉法结籽组合数提高34.09%,穗均结籽提高121.21%,出苗率提高60.07%,提高工效6-7倍;互花米草♀连续去雄授粉法比剪颖去雄授粉法结籽组合数增加3.14倍,穗均结籽增加4.21倍,出苗率提高68.47%,提高工效7-8倍。[结论]水稻♀热气去雄吸花授粉杂交法适用于水稻♀×互花米草♂;互花米草♀连续去雄授粉杂交法适用于互花米草♀×水稻♂。

英文摘要：

[Objective] This study aimed to explore germplasm resources of salt toler- ant Spartina alterniflora and high yield rice, and develop an innovative strategy for distant hybridization between S. altemiflora and rice. [Method] Crossing （rice ♀ ）： hot water emasculation and glume-cutting pollination method, heat emasculation and pollen-suction pollination method; reciprocal crossing （S. alterniflora ♀ glume-cutting emasculation and pollination method, continuous emasculation-pollination method, hot water emasculation and continuous emasculation-pollination method. [Result] In crossing groups （rice ♀ × S. alterniflora ♂ ）, compared with hot water emasculation and glume-cutting pollination treatment, the number of seed-bearing combinations, seed number per spike and seedling emergence rate in heat emasculation and pollen-suction pollination treatment were improved by 34.09%, 121.21% and 60.07%, respectively; hybridization efficiency was improved by 6-7 times. In reciprocal cross- ing groups （S. altemiflora ♀× rice ♂ ）, compared with glume-cutting emasculation and pollination treatment, the number of seed-bearing combinations and seed num- ber per spike in continuous emasculation-pollination treatment were improved by 3.14 and 4.21 times, respectively; seedling emergence rate was improved by 68.47%; hybridization efficiency was improved by 7-8 times. [Conclusion] Heat emas- culation and pollen-suction pollination method is suitable for hybridization between rice ♀× S. alterniflora ♂; continuous emasculation-pollination method is suitable for hybridization between S. alterniflora ♀× rice ♂.