中文摘要：

合成了高发光效率的CdSe/ZnS量子点并制备了CdSe/ZnS量子点-溶菌酶释放蛋白（MRP）抗体探针,利用凝胶电泳和分子光谱法研究了MRP抗体与CdSe/ZnS量子点的结合机理.荧光光谱法优化了CdSe/ZnS量子点-MRP抗体探针制备的影响因素,建立了一种测定MRP抗原的新方法,其线性范围为5.0×10^-8～1.5×10^-6mol/L,线性相关系数为0.9976,检测限为1.9×10^-8mol/L.

英文摘要：

High-quality CdSe/ZnS quantum dot and the CdSe/ZnS quantum dot-muramidase released protein （MRP） antibody probe were prepared, and the conjugation mechanism was studied according to the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE） and molecular spectrome- try. The factors influencing the preparation of CdSe/ZnS quantum dot-MRP antibody probe were optimized by fluorescence spectrometry. Under the optimum conditions, the probe showed good sensitivity to MRP antigen. The calibration graph was linear in the range from 5.0×10^-8 to 1.5×10^-6 mol/L of MRP antigen concentration, with a correlation coefficient of 0.9976 and a detection limit of 1.9×10^-8 mol/L.