千谷物的重量(TGWT ) 是在米饭影响谷物产量以及谷物质量的一个重要因素。为 TGWT 的量的特点地点(QTL ) qTGWT1-1 在在生来的线(RIL ) 人口从 indica-indica 米饭十字 Zhengshan97B (ZS97B )/Milyang46 (MY46 ) 导出的 recombinant 的染色体 1 的短手臂上在 DNA 标记 RG532 附近以前被检测。在这研究,二剩余异质接合的线(RHL ) , Ch1 和 Ch2,源于 ZS97B/MY46 RIL F-7 人口,被用来开发二张 F-6 人口, RIL-1 和 RIL-2。分别地, Ch1 和 Ch2 的染色体在染色体 1 的短手臂上由 RM1-RM3746 和 RM151-RM243 包含异质接合的区域 flanked,但是在另外的区域是同型结合的。与 TGWT 上的一样的添加剂方向和类似的效果,二紧连接的 QTL, Gw1-1 和 Gw1-2 在人口 RIL-2 在 QTL qTGWT1-1 的区域被检测。没有 QTL 在人口 RIL-1 被检测。分别地,从由 RM151-RM10404, RM10381-RM243, RM10435-RM259 和 RM10398-RM5359 带异质接合的片断 flanked 的人口 RIL-2 的四单个 RHL 被选择开发四张 F-2 人口。十母亲的同质接合体和 10 父亲的同质接合体从每人口从四 RHL 导出的四 F2 被选择。近的 isogenic 线(无) 的四个集合为 TGWT 的 phenotyping 和 Gw1-1 和 Gw1-2 的定界线被种。结果证明 Gw1-1 和 Gw1-2 位于分别地盖住 392.9 和 308.5 个 kb 区域的间隔 RM10376-RM10398 和 RM10404-RM1344。提高的等位基因从在两 loci 的 ZS97B,并且没有重要相互作用被检测。Gw1-1 和 Gw1-2 的基因解剖为他们在米饭克隆并且分子的繁殖谷物产量和质量打了一个基础。
Thousand-grain weight (TGWT) is an important factor affecting grain yield as well as grain quality in rice. A quantitative trait locus (QTL) qTGWTI-1 for TGWT was detected previously near DNA marker RG532 on the short arm of chromosome 1 in a recombinant inbred line (RIL) population derived from the indlca-indica rice cross Zhengshan97B (ZS97B)/Milyang46 (MY46). In this study, two residual heterozygous lines (RHLs), Chl and Ch2, derived from the ZS97B/MY46 RIL F7 population, were used to develop two Fe populations, RIL-1 and RIL-2. The genome of Chl and Ch2 contains a heterozygous region flanked by RM1--RM3746 and RM151--RM243 on the short arm of chromosome 1, respectively, but is homozygous in other regions. Two tightly linked QTLs, Gwl-1 and Gwl-2, with the same additive direction and similar effect on TGWT, were detected in the region of QTL qTGWTI-1 in population RIL-2. No QTL was detected in the population RIL-1. Four individual RHLs from the population RIL-2 carrying heterozygous segments flanked by RM151--RM10404, RM10381--RM243, RM10435--RM259 and RM10398--RM5359, respectively, were chosen to develop four F= populations. Ten maternal homozygotes and 10 paternal homozygotes were selected from each of the four F2 populations derived from the four RHLs. The four sets of near isogenic lines (NILs) were grown for phenotyping of TGWT and delimitation of Gwl-1 and Gw1-2. Results showed that Gwl-1 and Gw1-2 were located in the intervals RM10376--RM 10398 and RM10404--RM 1344 which cover 392.9 and 308.5 kb regions, respectively. The enhancing alleles were from ZS97B at both loci, and no significant interactions were detected. Genetic dissection of Gwl-1 and Gwl-2 has laid a foundation for their cloning and molecular breeding of grain yield and quality in rice.