中文摘要：

目的探讨神经病理性痛大鼠伏核注射甘丙肽的镇痛作用及其对星形胶质细胞活化的影响。方法左侧坐骨神经结扎构建神经病理性痛大鼠模型,伏核注射甘丙肽,测定伤害性热刺激和压力刺激诱发的后爪缩爪潜伏期（HWL）,观察甘丙肽的镇痛作用;Western bolt法检测星形胶质细胞的特异性标志物-胶质原纤维酸性蛋白（GFAP）的表达,观察伏核注射甘丙肽对星形胶质细胞活化的影响。结果左侧坐骨神经结扎引起大鼠双侧HWL缩短,且伏核GFAP的表达增加;神经病理性痛大鼠伏核注射甘丙肽引起双侧HWL延长,GFAP的表达减少。结论伏核微量注射甘丙肽对神经病理性痛的镇痛作用可能通过抑制星形胶质细胞的活化来完成。

英文摘要：

Objective To research the analgesic effect of nucleus accumbens（NAc）injection of galanin in rats with neuropathic pain and its influence on astrocyte activation.Methods The rat left sciatic nerve was ligated for constructing the neuropathic pain rat model.Galanin was injected by intra-NAc.The rat hindpaw withdrawal latency（HWL）induced by the damaging thermal and pressure stimuli was determined for researching the analgesic effect of galanin;the expression of glial fibrillary acidic protein（GFAP）as the astrocyte specific marker was detected by Western blot.The influence of NAc injection on the astrocyte activation was investigated.Results The bilateral HWL induced by the left sciatic nerve ligation was shortened,moreover the GFAP expression in NAc was upregulated;the intra-NAc injection of galanin induced an increase in bilateral HWL and a downregulation of GFAP expression.Conclusion The NAc microinjection of galanin may play the analgesic role on neuropathic pain via inhibiting the astrocyte activation.