中文摘要：

瞄准：为了学习肝房间 apoptosis，由硒的毒性引起了并且高用在试管内 9.4T 观察胆碱混合物的改变分辨率磁性的回声光谱学。方法：二十只男 Wistar 老鼠随机被划分成二个组。在治疗组的老鼠 intraperitoneally 与蒸溜水与钠亚硒酸盐和控制组被注射。所有老鼠被牺牲，肝被把。1H 太太数据高用在试管内 9.4T 被收集分辨率磁性的回声分光计。系列用 XWINNMR 和 MestRe-c 4.3 被处理。他和染色的 TUNEL 被雇用检测并且证实肝细胞的变化。结果：从老鼠的肝织物的高氯酸摘录的好 1H 先生系列被获得。常规代谢物被检测并且分配。在处理组对控制组的不同成分胆碱混合物的集中如下：全部的胆碱混合物， 5.08 +/- 0.97 mmol/L 对 3.81 +/- 1.16 mmol/L (P = 0.05 ) ；并且免费胆碱， 1.07 +/- 0.23 mmol/L 对 0.65 +/- 0.20 mmol/L (P = 0.00 ) 。然而，在二个组之间没有统计意义。肝的湾穴和在处理组的肝细胞的细胞的结构是反常的。肝细胞的 Apoptosis 被 TUNEL 试金证实。结论：高剂量硒混合物能引起老鼠肝损害和 induce 房间 apoptosis 在活体内。高分辨率 1H 太太在试管内能检测多样化的新陈代谢。为胆碱混合物的不同成分的变化趋势不是完全在 apoptosis 的早时期的一样。

英文摘要：

AIM： TO study liver cell apoptosis caused by the toxicity of selenium and observe the alteration of choline compounds using in vitro 9.4T high resolution magnetic resonance spectroscopy. METHODS Twenty male Wistar rats were randomly divided into two groups. The rats in the treatment group were intraperitoneally injected with sodium selenite and the control group with distilled water. All rats were sacrificed and the livers were dissected. 1H-MRS data were collected using in vitro 9.4T high resolution magnetic resonance spectrometer. Spectra were processed using XWINNMR and MestRe-c 4.3. HE and TUNEL staining was employed to detect and confirm the change of liver cells. RESULTS： Good 1H-MR spectra of perchloric acid extract from liver tissue of rats were obtained. The conventional metabolites were detected and assigned. Concentrations of different ingredient choline compounds in treatment group vs control group were as follows： total choline compounds, 5.08 ± 0.97 mmol/L vs 3.81±1.16 mmol/L （P = 0.05）; and free choline, 1.07 ± 0.23 mmol/L vs 0.65± 0.20 mmol/L （P = 0.00）. However, there was no statistical significance between the two groups. The hepatic sinus and cellular structure of hepatic cells in treatmentgroup were abnormal. Apoptosis of hepatic cells was confirmed by TUNEL assay. CONCLUSION High dose selenium compounds can cause the rat liver lesion and induce cell apoptosis in vivo. High resolution 1H-MRS in vitro can detect diversified metabolism. The changing trend for different ingredient of choline compounds is not completely the same at early period of apoptosis.