中文摘要：

目的 研究昆明小鼠眨眼条件反射延迟/痕迹任务模型建立过程中听觉惊吓反射（ASR）与眨眼条件反射（CR）的行为学特征参数与变异.方法 15只健康昆明小鼠随机分成3组：单任务延迟眨眼条件反射（DEBC）组（n=5）;单任务痕迹眨眼条件反射（TEBC）组（n=5）;双任务延迟/痕迹组（n=5）.采用不同音频（ TEBC 2KHz,DEBC1KHz）的正弦波纯音作为条件刺激（CS）,声音强度为85 dB.以100ms 束状氧气流作为非条件刺激（US）进行CS-US配对训练,观察实验各组行为学参数.结果 经过10d配对训练,惊吓反射率（ASR％）：单任务组[第10天ASR％分别达到（38.00±8.64）％和（38.55 ± 12.41）％,时间效应F=4.574,P=0.008;组间效应F=2.021,P=0.193））与双任务组[第10天ASR％分别达到（47.95±14.23）％和（62.01 ±9.03）％,时间效应F=5,547 P=0.013;组间效应F=0.738,P=0.415））均随训练天数增加显著增加.习得率（CR％）：单任务组[第10天CR％分别达到（45.4±5.39）％和（65.6±6.77）％,时间效应F=9.558,P=0.000;组间效应（F=5.117,P=0.054）与双任务组（第10天CR％分别达到（57.66±4.34）％和（77.35±7.36）％,时间效应F=7.750 P=0.002组间效应F=1.449 P=0.263））均随训练天数增加显著增加,两种模式间习得率未见明显差异.峰幅度（AP）：DEBC组随训练天数增加而增加（时间效应F=2.679,P=0.017）,双任务组随训练天数增加而增加（时间效应（F=3.452,P=0.034）.峰潜伏期（TP）无任何显著差异.结论 昆明小鼠可以成功建立单任务与延迟/痕迹双任务眨眼条件反射,虽然行为参数表现出一定程度的变异,但采取相关措施可予以控制.

英文摘要：

Objective To investigate the characteristic parameters and variation of acoustic startle reflex （ASR） and conditioning response （CR）during the acquisition of delay-trace eyeblink conditioning.Methods 15healthy Kunming mice were randomly divided into 3 groups：single-task DEBC group （ n =5 ） ; single-task TEBC group （ n =5） ; dual delay/trace group （ n =5）.Three groups received paired training of tone conditioned stimulus（CS）in different audio frequency （TEBC groups：2KHz; DEBC group：1KHz） and a 100 ms comeal oxygenpuff unconditioned stimulus（US）.Then observed the characteristic parameters of ASR and CR.Results After pairing training for 10 days,ASR rate（ ASR% ） of single-task groups （ 10th day （38.00 ± 8.64） % and （38.55 ±12.41 ） % respectively,time effect：F =4.574,P =0.008 ; group effect：F =2.021,P =0.193 ） and dual delaytrace group （ 10th day （47.95 ± 14.23 ） % and （62.01 ± 9.03 ） % respectively,time effect：F =5,547 P =0.013 ;group effect：F =0.738,P =0.415） changed significantly with the following training but between single-task groups or dual delay-trace group.CR rate （ CR% ） of single-task groups （ 10th day were （45.4 ± 5.39） % and （65.6 ± 6.77） % respectively,time effect：F =9.558,P =0.000 ; group effect：F =5.117,P =0.054 ） and dual delay/trace group （ 10th day （57.66 ± 4.34） % and （77.35 ± 7.36） % respectively,time effect：F =7.750,P =0.002 ;group effect：F=1.449,P=0.263 ） showed obvious change with the following training but between singletask groups or dual delay-trace group.Peak amplitude of single-task DEBC group （ time effect F =2.679,P =0.017 ） and dual delay-trace group （ F=3.452,P=0.034 ） had increased significantly with the following training.Peak latency showed no obvious change.Conclusion Dual delay-trace conditioning procedure of classical eyeblink conditioning and single-task can be successfully established in Kunming mice.Although there are some variations,those can be cont