中文摘要：

ObjectiveTo 在有 10 件批 SHLI 样品的过敏反应的 reaction.MethodsChemical 指纹的相互关联由高效液体层析(HPLC ) 是坚定的，并且进一步由类似分析调查了的 Shuanghuanglian 注射(SHLI ) 识别成分。与光显微镜学，两个过敏反应的实验和确定的试金结合了在老鼠 basophil 白血病房间(RBL-2H3 ) 被显示获得由 SHLI 导致的组织安版本。组织安的内容被连接酶的免疫吸着剂试金方法测试。部分最少的广场回归(PLSR ) 方法和 HPLC-DAD-ESI-MS ⁿ 技术被进行在包含光谱的过敏反应的 reaction.ResultsThe 结果的 SHLI 分析成分，效果关系证明八成分断然与过敏反应的反应被相关。在哪个之中，他们中的将近 90% 个与 PLSR 和 HPLC-DAD-ESI-MS ⁿ 。这结果根据 RBL-2H3 cells.ConclusionBaicalin 上的确定的试金，从 SHLI 的卢丁是包含过敏反应的反应的主要成分。

英文摘要：

OBJECTIVE： To identify the constituents in Shuanghuanglian injection（SHLI） that correlate with anaphylactoid reaction.METHODS： Chemical fingerprints of 10 batches SHLI samples were determined by High Performance Liquid Chromatography（HPLC）, and further investigated by similarity analysis. Combined with optical microscopy, both anaphylactoid experiments and confirmatory assay were displayed in Rat basophil leukemia cells（RBL-2H3） to obtain the histamine release inducing by SHLI. The content of histamine was tested by Enzyme-Linked Immuno Sorbent As-say method. Partial least squares regression（PLSR）method and HPLC-DAD-ESI-MSntechnology were conducted to analyze constituents in SHLI involving anaphylactoid reaction.RESULTS： The results of spectrum and effect relationships showed that the eight constituents were positively correlated with anaphylactoid reaction.Among which, nearly 90% of them were identified as baicalin and rutin with PLSR and HPLC-DAD-ESI-MSn. This result was in accordance with confirmatory assay on RBL-2H3 cells.CONCLUSION： Baicalin and rutin from SHLI were the main constituents involving anaphylactoid reaction.