中文摘要：

目的探讨异氟醚预处理对大鼠肝脏缺血再灌注损伤的影响。方法成年雄性SD大鼠24只，体重180～220g，随机分为3组（n=8）：假手术组（S组）吸入纯氧30min，间隔30min后仅开腹；肝脏缺血再灌注组（IR组）吸入纯氧30min，间隔30min后行肝脏缺血60min，再灌注4h；异氟醚预处理组（Iso组）吸入1．4％异氟醚30min，间隔30min后行肝脏缺血60min，再灌注4h。于再灌注4h时处死大鼠，留取肝脏及腹主动脉血5ml。测定血清谷丙转氨酶（ALT）和谷草转氨酶（AST）浓度，血清及肝组织匀浆上清液中肿瘤坏死因子α（TNF-α）的浓度，肝组织髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量，观察肝组织病理学改变。结果与S组比较，IR组、Iso组血清ALT、AST和TNF-α水平明显升高，肝组织TNF—α含量升高，肝组织MPO活性升高，MDA含量升高，SOD活性降低（P〈0．05或0．01），肝组织病理损伤明显；与IR组比较，Iso组血清ALT、AST和TNF-α水平降低，肝组织，TNF-α含量降低，肝组织MPO活性降低，MDA含量降低，SOD活性升高（P〈0．05或0．01），肝组织病理损伤程度减轻。结论1．4％异氟醚预处理可明显减轻大鼠肝脏缺血再灌注损伤，其机制可能与抑制TNF-α的释放、减少中性粒细胞在肝组织的浸润有关。

英文摘要：

Objective To investigate the effects of isoflurane preconditioning on hepatic ischemiareperfusion injury in rats. Methods Twenty-four adult male SD rats weighing 180-220 g were randomly divided into 3 groups （ n = 8 each） ： group Ⅰ sham operation （S） ; group Ⅱ I/R and group m iso. The animals were anesthetized with intraperitoneal 3 % pentobarbital 40 mg/kg. Partial liver ischemia was produced by clamping the hepatic arteries supplying left lobe and middle lobe and portal vein for 60 min with atraumatic mini-clamp, followed by 4 h of reperfusion in I/R and Iso groups. In Iso group the animals inhaled 1.4% isoflurane for 30 min at 30 min before I/R. The animals were killed at 4 h of reperfusion. The liver was removed and 5 ml blood was taken from abdominal aorta for determination of （1） serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST） activities ; （2） liver TNF- α content and serum TNF-α concentration ; （3） myeloperoxIdase （MPO） and SOD activities and MDA content in the liver and （4） microscopic examination. Results The serum ALT, AST and TNF-α levels; the MPO activity and MDA and TNF-α contents in the liver were significantly higher and SOD activity in the liver was significantly lower after hepatic I/R in I/R and Iso groups than in S group. Isoflurane preconditioning significantly ameliorated the deleterious effects of I/R and I/R-induced liver tissue damage. Conclusion Preconditioning with 1.4% isoflurane can significantly ameliorate hepatic I/R injury by inhibiting TNF-α release and reducing neutrophil infiltration in liver tissue.