中文摘要：

目的 通过观察调中汤对脾虚肝旺型功能性消化不良幼鼠胃窦胃动素（MTL）、血清干细胞因子（SCF）的影响,探讨调中汤对脾虚肝旺型功能性消化不良的具体作用机制。方法 选取清洁级SD幼鼠36只,日龄21～28d,体重50～70 g,随机分为6组,每组6只：空白对照组、模型组、调中汤高剂量组、调中汤中剂量组、调中汤低剂量组、多潘立酮组（西药组）。幼鼠在购入后适应环境喂养1周,正常喂养,第2周开始,除对照组每日常规饲养不予造模外,其余组采用不规则喂养、饮用4℃冰水及夹尾刺激法造模。造模成功后,从造模后第7天开始用药,持续给药14 d。各组幼鼠在末次给药前12 h禁食（自由饮水）,腹腔静脉取血,分离血清,采用ELASE法用酶标分析仪检测SCF;取胃窦的胃壁全层一块,用免疫组化法测定MTL。结果 调中汤高、中剂量组均能提高幼鼠血清SCF含量,与模型组比较差异有统计学意义（P〈0.01）,调中汤高剂量组可以增加幼鼠胃窦MTL含量,与模型组比较差异有统计学意义（P〈0.01）,中剂量组与模型组比较差异有统计学意义（P〈0.05）。结论 成功建立脾虚肝旺型功能性消化不良幼鼠模型,并观察到调中汤能明显提高脾虚肝旺型功能性消化不良幼鼠血清SCF及胃窦MTL水平,说明上调MTL、SCF表达是调中汤治疗脾虚肝旺型FD的作用机制之一。

英文摘要：

Objective To study the impact of Tiaozhong decoction on motilin（MTL） and Stem Cell Factor（SCF） levels in the spleen deficiency with liver hyperactivity young rats with functional dyspepsia（FD） , and discuss the possible mecha- nism of action. Methods Total 36 clean grade SD young rats, age 21 - 28 d,weighing 50 - 70 g, were randomly divided into 6 groups ： the normal control group, the model control group, the high dose group of Tiaozhong decoction, the middle dose group of TiaoZhong decoction, the low dose group of TiaoZhong decoction and the domperidone group,with 6 rats in each group. These young rats were fed regularly on the 1 th week to adapt to the environment, then from the 2nd week, the rats （except for the normal control group） were fed irregularly, administrated with ice water（4 ℃ ） and received tail clamp stimulation to develop a rat model. The drugs were administered continuously for 14 days after the model was successfully established. The young rats in each group were fed nothing except water for 12 hours before the administration of medi- cine. Then, blood samples were collected from abdominal aortic and the he levels of SCF were tested by ELASE method. Afull thickness of gastric antrum wall was sampled to determine the MTL level by using immunohistochemistry method. Results The SCF level in serum of the high and middle dose group were significantly higher than that in the model group （P 〈0.01 ） ;The MLT level of the high dose group was significantly higher than that in the model group（P 〈 0.01 ） ,the levels between the middle dose group and the model group was statistically significant （P 〈 0.05）. Conclusion The model rat was successfully developed. Tiaozhong decoction can significantly improve the level of SCF in serum and MTL in gastric antrum. The up-regulation of MT and SCF may be the proper mechanism of Tiaozhong decoction in the treatment of spleen-deficiency with liver-hyperactive young rats with functional dyspepsia.