中文摘要：

为了研究外源信号分子对高表达玉米C4型pepc酶活性的调节机制，本实验以高表达玉米C。型pepc水稻（PC）和原种水稻Kitaake（岍）的悬浮细胞系为材料，研究葡萄糖、胡蜂蜂毒肽（mastoparan，Mas）及其磷脂酸（phosphatidic acid，PA）的两个合成途径的专一性抑制剂（磷脂酶D（phospholipaseD，PLD）的专一一肚抑制剂正丁醇和磷脂酶C（phospholipaseC，PLC）的专一性抑制剂新霉素）对供试材料PEPC活性的影响。结果表明，3％葡萄糖处理0．5h对、vT的PEPC酶活性抑制最明显；相对于PC，则5％的葡萄糖处理5h时，埘其PEPC酶活性抑制的最明显。5mmol／L的Mas对wT和PC悬浮细胞的PEPC酶活性分别抑制了约60％和40％。0．01mmol／L、0．1mmol／L和1mmol／L新霉素处理对WT的PEPC酶活性影响不明显，且无时间效应；与wT相比较，不同浓度的新霉素均增强了PC的PEPC酶活性，其中0．1mmol／L新霉素处理增加为对照的约7倍。正丁醇处理明显抑制了WT的PEPC酶活性，但不同浓度处理之间却没有显著筹异；而对rPC，0．02％、0．04％正丁醇处理使得PEPC酶活性显著下降，且比wT的更显著。同时0．04％的止丁醇的抑制效应具有明显的时间效应。以上结果表明，对PC的PEPC酶活性的调节主要依赖于由PLD途径产生的PA的参与。

英文摘要：

In order to investigate the regulatory mechanism of external signaling molecules on the over-expressing C4-pepc activity, suspension cell lines of over-expressing maize pepc （PC） and Kitaake （WT） were used as materials, and the effects of glucose, mastoparan （Mas） and the two specificity inhibitors of phosphatidic acid （PA） synthesis methods （1-butanol, the specificity inhibitor ofphospholipase D （PLD）; and neomycin, the specificity inhibitor ofphospholipase C （PLC）） on PEPC activity were investigated at the cellular level. The results indicated that there was an obvious inhibition on PEPC activity of WT with the treatment of 3% glucose for 0.5 h; for PC, there was the most notable inhibition with 5% glucose treatment for 5 h. The PEPC activity of both WT and PC could be sup- pressed by 60% and 40% with the 5 mmol/L Mas treatment, respectively. There was no obvious effects on the PEPC activity of WT suspension cells at the different concentrations of neomycin, neither did the effects by time; Compared to WT, the PEPC activity of PC suspension cells was enhanced by neomycin and it was nearly 7 times of controls with 0.1 mmol/L neomycin treatment. The PEPC activity of WT was significantly suppressed by 1-butanol, while it did not have remarkable differences among different concentrations; for PC suspension cells, the treatments of 0.02% and 0.04% 1-butanol made the PEPC activity decreased, which were more obviously than WT. Meanwhile, the inhibition of PEPC activity under the treatment of 0.04% 1-butanol also increased with the treated time. All above results suggest that the regulation to PEPC activity of PC is mainly dependent on the participation of PA by PLD.