利用燕麦-琼脂培养、基物培养及扫描电镜技术研究了细弱绒泡菌的个体发育过程,在燕麦琼脂培养基上完成了从孢子到孢子的生活史。结果表明,细弱绒泡菌生活史包括单核的黏变形体或游动胞、多核的营养体原质团以及孢子形成阶段。孢子球形,表面具细小疣点。孢子萌发为裂式,释放1黏变形体。黏变形体行变形运动,在有水的条件下,可转变为游动胞。成熟原质团橘黄色。原质团类型为显型,具有扇形网络状菌脉。成熟原质团可形成多个孢囊。琼脂培养基上获得的细弱绒泡菌孢子与野生型相似,并具有可育性。
Oat-agar culture method, moist chamber culture method and scanning electron microscopy were used to study the process of the individual development of Physarum tenerum. The life cycle of spore-to-spore were completed in agar culture in Petri dish. The result shows that the life cycle of Physarum tenerum comprises a unicellular amoeboid or swarm cell stage, a multinucleate plasmodium stage and a sporulation stage. The spore of Physarum tenerum is globose and the spore surface is minutely spiny. The spore germinates by means of a crack open and releases a single myxamoeba. The myxamoeba can move by amoeboid motion. When free water is available, myxamoebae can transform into swarm cells. The mature plasmodium is orange. The plasmodial type is the phaneroplasmodium which appears as a fan-shaped network of veins. The mature plasmodium can form many sprangia. Spores developed on agar are fertile and resemble those on natural substrate.