中文摘要：

为了探索双峰驼黄体细胞的percoll液分离及体外培养方法，观察黄体细胞的生物学特性，采用胶原酶消化法从双峰驼黄体组织中分离并获得妊娠期双峰驼黄体细胞，通过形态学观察确定了大小黄体细胞的生长特性．结果表明：胶原酶消化可获得数量较多，生长状态良好的双峰驼黄体细胞，而小黄体细胞的数量远远多于大黄体细胞，其形态学和生物学特性具有典型类固醇分泌细胞的特征．试验建立的体外双峰驼黄体细胞原代培养的方法，所获细胞生物学特征稳定，纯度较高，适用于体外试验研究．

英文摘要：

This study aims to establish a method of isolation and primary culture of corpus luteum cells from Bactrian camel during pregnancy by using percoll,in order to observe the biological characteristics of large luteal cells and small luteal cells. Luteal cells were obtained by collagenase digestion from corpus lute- um of pregnant Bactrian camel. The results showed that the majority of Bactrian camel corpus luteum cells with good growth status could be obtained by collagenase digestion, and the number of small luteal cells was more incredible than the number of large luteal cells. The amplification cells showed representative morphological and biological characteristics of steroid secretory cells. It is suggested that the method of iso- lation and primary culture of Bactrian camel luteal cells in vitro is established successfully. The luteal cells are suitable for experiment in vitro for its high purity and stable biological characteristics,and provided the basis for further research on corpus !uteum cells of Bactrian camel breeding mechanism.