中文摘要：

采用水培实验,研究了5个氮（N）浓度下（0.01-5 mmol L-1）水稻的生物量、体内氮浓度、根系发育、体内生长素浓度以及生长素外流蛋白OsPIN家族基因的表达情况。结果表明,与正常供氮水平（2.5mmol L-1）相比,低氮（0.01 mmol L-1）胁迫下水稻根冠比增加28%,地上部全氮浓度降低约20%,根系全氮浓度降低约33%,种子根长度增加25%,种子根上的侧根密度降低26%,倒一叶中的生长素含量增加140%,而根茎结合处和根系的生长素浓度分别下降22%和60%;RT-PCR的结果表明,低氮（0.01 mmol L-1）胁迫下水稻根系中OsPIN1a-b、OsPIN2、OsPIN5a-b和OsPIN9基因表达显著下调;而外源生长素α-萘乙酸（NAA）和生长素极性运输抑制剂1-萘氨甲酰苯甲酸（NPA）的施加均能影响到水稻种子根长和种子根上的侧根密度。由此推论,低氮胁迫下水稻体内生长素从倒一叶到根系极性运输减少是水稻根系对低氮胁迫响应的生理机制之一。

英文摘要：

The ability of plants to sense available nutrients in the soil and to respond accordingly is of fundamental importance for their adaptation to the environment. The plasticity of root in development in response to nitrogen（ N） deficiency is vital,as N is a major nutrient essential for plant growth and development. Changes in root morphology under the stress of N deficiency are complex and vary with experimental conditions and plant species. Little attention has been paid to root growth under the stress of N deficiency,possibly because of the inconsistent response of primary root in length to N deprivation depending on plant age and N concentration supplied. Thus root morphology in rice under the stress of N deficiency has not yet been characterized in detail. Root formation is regulated by both environmental conditions and intrinsic factors. Auxins play a key role in rice plants establishing and developing root morphology. Few studies have evaluated the role of auxins in regulating root growth under low N conditions. To what extent,if any,auxin transport in rice roots is regulated by N deficiency remains unclear. In this paper,a hydroponic media experiment was carried out on N concentration regulating auxin transport and relationship between root formation and transport and distribution of auxins in rice.In this study,biomass,N concentration,seminal root length and lateral root density（ LR density） of rice plants and auxin concentration in the plant and relative expression of OsPIN family genes of auxin outflowing protein were investigated relative to N concentration（ 0. 01,0. 2,1,2. 5 and 5 mmol L- 1） in hydroponic media. Comparison of the plants under two N concentrations（ 0. 01 and 2. 5 mmol L- 1） reveals that the ratio of root to shoot increased significantly with decreasing N concentration,partly resulting from decline of the plant root in biomass relative to shoot. However,compared with the plants under normal N concentration（ 2. 5 mmol L- 1）,the plants under low N concentration（ 0. 01