中文摘要：

目的：探查伤寒沙门菌mig-14在高渗应激下对若干基因表达的调节作用。方法：用自杀质粒介导的同源重组方法制备伤寒沙门菌mig-14基因缺陷变异株,用基因芯片分析高渗应激早期野生株和mig-14缺陷株基因表达差异,并对部分结果采用实时荧光定量PCR进行验证。结果：成功制备伤寒沙门菌mig-14缺陷株,高渗应激早期mig-14变异株与野生株相比有77个基因表达下调和72个基因表达上调。结论：mig-14可能是一种调节基因,主要参与调节细菌的物质和能量代谢。

英文摘要：

Objective： To explore the function of mig-14 of Salmonella enterica serovar Typhi in response to hyperosmotic stress. Methods： A mig-14 gene deleted mutant of S. enterica serovar Typhi was constructed using the homologous recombination method by means of a suicide plasmid; the difference of the gene expression between the wild strain and the mig-14 mutant at early-stage of hyperosmotic stress was investigated by genomic microarray assay and partially confirmed by real time quantity reverse transcription PCR. Resuits： Successfully constructed a mig-14 mutant; about 77 and 72 genes were down-and up-regulated respectively at early-stage of hyperosmotic stress in mig-14 mutant. Conclusion： mig-14 may be a regulator, may primally regulate the substance metabolism and energy metabolism of Salmonella enterica.