中文摘要：

通过选择性抽提经环六亚甲基双乙酰胺（hexamethylene bisacetamide，HMBA）诱导处理前后的人肝癌SMMC-7721细胞核基质，并运用亚细胞蛋白质组学等分析技术，研究nucleophosmin（NPM）在核基质上的表达和定位变化，及其与相关基因产物的共定位关系，观察研究了nucleophosmin在诱导分化前后人肝癌SMMC-7721细胞核基质中的存在、分布及其与相关基因产物的共定位关系．双向凝胶电泳和质谱鉴定结果显示，nucleophosmin存在于SMMC．7721细胞核基质蛋白组分中，在HMBA处理后细胞核基质中表达下调．蛋白质印迹杂交实验结果确证了nucleophosmin在核基质中的存在及其在诱导处理后细胞核基质中表达下调的变化．免疫荧光显微镜观察显示，nucleophosmin定位在SMMC-7721细胞核基质上，经HMBA处理后出现分布位置与表达水平的变化．激光扫描共聚焦显微镜观察结果显示，SMMC-7721细胞中，nucleophosmin与c-fos、c-myc、rb、p53等基因产物具有共定位关系，但在诱导处理后细胞内的共定位区域发生了改变．研究结果证实，nucleophosmin是一种核基质蛋白，定位于核基质纤维上，nucleophosmin在人肝癌SMMC-7721细胞诱导分化过程中的表达分布，及其与相关癌基因、抑癌基因产物的关系对SMMC．7721细胞分化具有重要影响．

英文摘要：

To explore the existence and distribution of nucleophosmin in the nuclear matrix and its colocalization with the other related gene products following HMBA treatment in the human hepatocarcinoma SMMC-7721 cells, the nuclear matrix of SMMC-7721 cells was extracted pre/post HMBA induced differentiation.2D PAGE proteomics analyses showed that nucleophosmin existed in the fractions of nuclear matrix proteins and was down-regulated after HMBA treatment with further confirmation by Western blot analysis. The immunofluorescence observation revealed that nucleophosmin located in the nuclear matrix, HMBA treatment altered its expression level and distribution profile. The co-localization of nucleophosmin with cancerrelated genes and the products of oncogenes or tumor repression genes, including c-los, c-myc, p53 and Rb, using laser scanning confocal microscopy, were evaluated, and substantial differences were observed following HMBA treatment. The results implies that nucleophosmin, as a nuclear matrix protein, the level of its expression and the colocalization with cancer-related gene products SMMC-7721 cell. may play an important role during the differentiation of SMMC-7721 cell.