中文摘要：

目的探讨糖皮质激素对视网膜神经节细胞（retinal ganglion cell,RGC）凋亡的影响及可能机制。方法分4组培养RGCs,即①对照组,②激素组（0.1μmol/L可的松）,③激素-siNgR组（0.1μmol/L可的松＋NgR反义核苷酸病毒）,④激素-scRNA对照组（0.1μmol/L可的松＋阴性核苷酸病毒）。3d后四甲基噻唑蓝（Thiazolyl blue tetrazolium bromide,MTT）检测细胞活力变化,倒置显微镜观察细胞形态学变化,Hoechst 33342染色检测细胞凋亡,Western blot检测Nogo受体（Nogo receptor,NgR）表达。结果RGCs细胞活力在激素组（76.3±6.8）%和激素-scRNA组（79.4±9.0）%明显低于对照组（100.0±0.0）%和激素-siNgR组（96.7±9.8）%。但是,NgR表达水平在激素组和激素-scRNA组明显高于对照组和激素-siNgR组（P〈0.01）。Hoechst 33342染色显示,激素组及激素-scRNA组RGCs可见大量亮蓝色的凋亡细胞,但在对照组及激素-siNgR组未见凋亡细胞。结论糖皮质激素诱导RGC凋亡与上调NgR表达相关。

英文摘要：

Objective To study the effect and possible mechanism of glucocorticoid in the apoptosis of retinal ganglion cells（RGCs）.Methods RGCs were cultured and divided into 4 groups：control group,glucocorticoid group（0.1μ mol/L cortisone）,glucocorticoid-siNgR group（0.1μmol/L cortisone＋NgR antisense nucleotide）,glucocorticoid-scRNA group（0.1μmol/L cortisone＋scrambled nucleotide）.The cells viability was detected by MTT method,the morphology was observed with inverted microscope,apoptosis of RGCs was measured with hoechst 33342 staining,and expression of NgR was revealed by Western blot.Results The cell viability of RGCs was decreased in glucocorticoid（76.3 ± 6.8）%or glucocorticoidscRNA（79.4 ± 9.0）%groups than in control（100.0 ± 0.0）%or glucocorticoid-siNgR（96.7 ± 9.8）%groups,but the expression level of NgR in RGCs was increased in glucocorticoid or glucocorticoid-scRNA groups than in control or glucocorticoid-siNgR groups.The hoechst33342 staining showed a lot of bright blue apoptic RGCs in glucocorticoid and glucocorticoid-scRNA groups,but not in control and glucocorticoid-siNgR groups.Conclusion The induction of RGCs apoptosis by glucocorticoids might be related to the upregulation of NgR in RGCs.