中文摘要：

目的探讨血管内皮生长因子（vascular endothelial groth factor,VEGF）是否通过缝隙连接蛋白43（connexin 43,Cx43）调控内皮祖细胞（endothelial progenitor cells,EPCs）增殖迁移。方法分离培养原代大鼠脾源性EPCs,不同浓度VEPF（0、10、50 ng/m L）刺激细胞,Western blot检测Cx43的表达。实验分3组：对照组、VEGF组、VEGF＋si Cx43组。荧光漂白恢复实验（fluorescence redistribution after photobleaching,FRAP）分别检测3 min内各组选定细胞荧光强度变化情况。CCK-8检测干扰Cx43蛋白表达对EPCs增殖的影响;Transwell小室迁移实验检测干扰Cx43蛋白表达对EPCs迁移的影响。结果 （1）VEGF促进EPCs中Cx43的表达（P〈0.05）,且有浓度依赖性。（2）与对照组相比,VEGF组缝隙连接功能明显增强（P〈0.05）;与VEGF组相比,VEGF＋si Cx43组缝隙连接功能明显降低（P〈0.05）。（3）与对照组相比,VEGF组促进EPCs增殖及迁移,差异有统计学意义（P〈0.05）;与VEGF组相比,VEGF＋si Cx43组EPCs增殖及迁移活性明显降低（P〈0.05）。结论 VEGF可以通过上调EPCs中Cx43的表达,引起缝隙连接功能增强,从而促进内皮祖细胞的增殖及迁移。

英文摘要：

Objective To explore whether vascular endothelial growth factor（ VEGF） can regulate the proliferation and migration in endothelial progenitor cells（ EPCs） through connexin 43（ Cx43）. Methods Primary EPCs derived from mouse spleen were isolated and cultured,and different concentrations of VEGF（ 0,10,and 50 ng /m L） were used to stimulate EPCs. Western blotting was employed to detect the expression of Cx43. EPCs were divided into the control group, VEGF group, and VEGF ＋ si Cx43 group, and fluorescence redistribution after photobleaching（ FRAP） was adopted to detect the fluorescent intensity in the EPCs selected from each group in 3 min. The effect of Cx43 on the proliferation of EPCs was determined by CCK-8 assay. Transwell migration assay was used to analyze the effect of Cx43 interference on the migration of EPCs. Results VEGF promoted the expression of Cx43 in the EPCs in a concentration-dependent manner（ P〈0. 05）. Compared with the control group,the gap junction function of the VEGF group was enhanced significantly（ P〈0. 05）,while compared with the VEGF group,the gap junction function of the VEGF ＋si Cx43 group was weakened significantly（ P〈0. 05）. Compared with the control group,the VEGF group showed accelerated proliferation and migration of EPCs,and the differences were statistically significant（ P〈0. 05）. Compared with the VEGF group,the proliferation and migration of EPCs were decreased significantly in the VEGF ＋ si Cx43 group（ P〈0. 05）. Conclusion VEGF can increase the expression of Cx43 in EPCs,which enhances the gap junction function and thus promotes the proliferation and migration of EPCs.