中文摘要：

目的检测SLE患者外周血人巨细胞病毒（HCMV）的感染状况，探讨HCMV感染在SLE发病中的作用。方法采集并抽提60例已确诊的SLE患者和111名健康人外周血白细胞DNA，利用巢式PCR技术检测HCMV糖蛋白gB（UL55）基因以确定HCMV感染情况。应用2个独立样本t检验、非参数检验、χ2检验及Fisher确切概率法统计分析。结果琼脂糖凝胶电泳及测序显示建立的巢式PCR能特异地检测HCMV．UL55基因，SLE患者外周血中HCMV检出率明显高于对照组[分别为41．7％（25／60）和1．8％（2／111），χ2=46．551，P〈0．01]。与外周血HCMV阴性组SLE患者相比，HCMV阳性组SLE患者抗核糖体P蛋白抗体（Rib．P）阳性率[分别为26％（9／35），56％（14／25），χ2=5．659，P=0．017]、直接Coomb’s试验阳性率[分别为37％（13／35），72％（18／25），χ2=7．096，P=-0．008]及抗β2糖蛋白1（GP1）抗体水平[分别为21．3（9．9，51．8）U/ml，13．6（5．9，23．1）U／ml；U=2．017，P=0．044]明显升高，血液系统相关指标中的红细胞[分别为（3．65±0．10）×10^12／L，（3．17±0．17）×10^12／L；t=2．574，P=0．013]、淋巴细胞[分别为（1．37±0．14）×10^12／L，（0．90±0．13）×10^12；t=2．456，P=0．017]、血红蛋白[分别为（110±19）g／L，（98±5）g／L；t=2．034，P=0．048]明显减少；肾损害相关指标中的血尿阳性率[分别为40％（14／35），72％（18／25）；χ2=6．000，P=0．014]、尿蛋白定量（24h）水平[分别为0．80（0．53，2．37）g，0．48（0．13，1．21）g；U=2．140，P=0．032]明显上升。结论外周血细胞HCMV感染可能参与SLE发生、发展过程。

英文摘要：

Objective To detect the prevalence of human cytomegalovirus （HCMV） in peripheral blood of patients with systemic lupus erthematosus （SLE） and explore its role in the pathogenesis of SLE. Methods HCMV DNA was isolated from the peripheral blood leucocytes （PBLs） of 60 patients with SLE and 111 healthy controls. Nested polymerase chain reaction （nPCR） technology was used to investigate the gene of HCMV glycoprotein gB （UL55） in these specimens. HCMV infections in the PBLs of SLE patients were confirmed by HCMV-ULSS detection. Two-sample t test, nonparametric test, Chi-square test and Fisher probabilities were used to analyze. Results Agarose gel electrophoresis and sequencing analysis showed that established nPCR could specifically detect HCMV-UL55 gene, the HCMV infection rate was significantly higher in patients with SLE than in the healthy controls （P〈0.01）. Positive rates of HCMV infection in SLE group and controls were 41.7%（25/60） and 1.8%（2/111）, respectively. Compared to the SLE patients with HCMV-negative PBLs, the positive rate of Rib-P [26%（9/35） vs 56%（14/25）, χ2=5.659, P=0.017], the positive rate of direct Coomb＇s test [37%（13/35） vs 72%（18/25）, χ2=7.096, P=0.O08 ] and the level of anti-β2GP1 [21.3 （9.9,51.8） U/ml vs 13.6 （5.9,23.1） U/ml, U=2.017, P=0.044] were significantly higher than those in the SLE patients with HCMV-positive PBLs. Compared to the SLE patients with HCMV-negative PBLs, the number of red blood ceils [（3.65±0.10）x10±2/L vs （3.17±0.17）×10^12/L, t=2.574, P=0.013] and lymphocytes [（1.37±0.14）x10±2/L vs （0.90±0.13）×10^12/L, t=2.456, P=0.017] in peripheral blood and the hemoglobin levels [ （110±19） g/L vs （98±5）g/L, t=2.034, P=0.0481 of the SLE patients with HCMV-positive decreased significantly. At the same time, the positive rate of hematuria [40%（14/35） vs 72% （18/25）, ）（2= 6.000, P=0.014] and 24 h proteinuria [0.80 （0.53,2.37） g vs 0.48 （0.13,1.21） g, U=2.140, P=0.032],