以草鱼鱼鳞为原料,分男::用水提法和酶解法提取胶原蛋白,通过测定提取样品的胶原蛋白含量优化试验条件,并对两种鱼鳞胶原蛋白提取工艺进行比较研究。结果表明:同在最优试验条件下(水提法试验温度为70%,料液比为1:40(g:mL),反应时间为20min;酶解法试验温度为25℃,酶与底物比为3%,酶解时间为120min,pH为3),酶解法提取鱼鳞胶原蛋白得率明显要高于水提法,但酶解法试验成本高于水提法。
The extraction conditions of collagen from grass carp scale were optimized by measuring hydroxyproline content in the extraction sample, and the technique comparison between two extraction methods were carried out in the present work. Results showed that the optimum experiment conditions of water extraction were temperature 70℃, solid-liquid ratio 1:40 (g: mL) , reaction time 20 min; and the optimum experiment conditions of pepsin extraction were temperature 25℃, enzyme to substrate ratio 3% , hydrolysis time 120 min, and pH 3. As a result, the production by pepsin extraction method was higher than that from water extraction method, however, the cost of the former was also higher.